PE/Cyanine7 anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
publications
2B8_PECy7_042408
C57BL/6 bone marrow cells stained with B220 FITC and 2B8 PE/Cyanine7
  • 2B8_PECy7_042408
    C57BL/6 bone marrow cells stained with B220 FITC and 2B8 PE/Cyanine7
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
105813 25 µg 64€
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105814 100 µg 156€
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1 and immunohistochemistry of acetone fixed frozen sections2. The 2B8 antibody does not block c-Kit activity.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
Product Citations
  1. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  2. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  3. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  4. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  5. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  6. Katsumura KR, et al. 2018. Proc Natl Acad Sci U S A. 115:E10109. PubMed
  7. Mantri CK, et al. 2019. J Clin Invest. 129:1094. PubMed
  8. Soukup AA, et al. 2019. J Clin Invest. 129:1180. PubMed
  9. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  10. De S, et al. 2018. Development. 145. PubMed
  11. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  12. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  13. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  14. Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed
  15. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  16. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  17. Dráberová L, et al. 2020. Front Immunol. 10:3068. PubMed
  18. Simon M, et al. 2019. Cell Metab. 29:871. PubMed
  19. Ray S, et al. 2020. J Biol Chem. 295:7113. PubMed
  20. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  21. Wilkinson AC, et al. 2019. Nature. 571:117. PubMed
  22. Shibata F, et al. 2009. Stem Cells. 27:183. PubMed
  23. Shiozawa Y, et al. 2010. PLoS One. 5:e10853. PubMed
  24. Joseph J, et al. 2012. Mol Cancer Res. 0.681944444. PubMed
  25. Xu H, et al. 2012. Blood. 120:3089. PubMed
  26. Nakano N, et al. 2008. J Allergy Clin Immunol. 123:74. PubMed
  27. Obata-Ninomiya K, et al. 2013. J Exp Med. 210:2583. PubMed
  28. Kanemaru K, et al. 2015. PLoS One. 10: 0144436. PubMed
  29. Chen I, et al. 2016. Sci Rep. 6:27195. PubMed
  30. Zheng X, et al. 2015. Cell Rep. 13:1505-1518. PubMed
  31. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
  32. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  33. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  34. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  35. Yao Z, et al. 2020. Cancer Res. 80:1171. PubMed
  36. Sulsenti R, et al. 2021. Frontiers in Immunology. 12:622001. PubMed
  37. Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
  38. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  39. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  40. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  41. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  42. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  43. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
RRID
AB_313222 (BioLegend Cat. No. 105813)
AB_313223 (BioLegend Cat. No. 105814)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.28.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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