PE/Cyanine7 anti-human/mouse CXCR7 Antibody

Pricing & Availability
Clone
8F11-M16 (See other available formats)
Regulatory Status
RUO
Other Names
Chemokine (C-X-C motif) receptor 7, RDC1, CMKOR1, GPR159
Isotype
Mouse IgG2b, κ
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Product Citations
publications
8F11-M16_PECy7_CXCR7_Antibody_FC_050515
CXCR7 transfected L1.2 cells were stained with CXCR7 (clone 8F11-M16) PE/Cyanine7 (filled histogram) or mouse IgG2b, κ PE/Cyanine7 isotype control (open histogram).
  • 8F11-M16_PECy7_CXCR7_Antibody_FC_050515
    CXCR7 transfected L1.2 cells were stained with CXCR7 (clone 8F11-M16) PE/Cyanine7 (filled histogram) or mouse IgG2b, κ PE/Cyanine7 isotype control (open histogram).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
331115 25 tests 132,00€
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331116 100 tests 268,00€
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Description

CXCR7, also known as RDC1, belongs to a subgroup of C-X-C chemokine receptors, which are part of a large protein family of G protein-coupled receptors (GPCR). CXCR7 binds with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC, which regulates the trafficking and activation of leukocytes. It is also a co-receptor for the entry of HIV-1. Binding of ligand to CXCR7 induces proliferation and migration of immature neurons, glia and their precursors. CXCR7 expression occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. Surface expression of CXCR7 was also reported to be on tumor cells, activated endothelial cells, fetal liver cells, and other cell types.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CXCR7 transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICFC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Intracellular staining for flow cytometry.2

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Soehnlein O, et al. 2013. EMBO Mol. Med. 5:471. (FC) PubMed
  2. Saad ST, et al. 2014. PLoS One 9(1). (ICFC) PubMed
  3. Balabanian K, et al. 2012. J. Transl. Med. 10:251. (FC) PubMed
Product Citations
  1. Ceholski D, et al. 2017. Stem Cell Res. 10.1016/j.scr.2017.06.015. PubMed
RRID
AB_2565678 (BioLegend Cat. No. 331115)
AB_2565679 (BioLegend Cat. No. 331116)

Antigen Details

Structure
G protein-coupled receptor super family
Distribution

Monocytes, B cells, T cells and mature dendritic cells

Ligand/Receptor
CXCL12/SDF-1 and CXCL11/I-TAC
Cell Sources
On a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells
Cell Type
B cells, Dendritic cells, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Burns JM, et al. 2006. J. Exp. Med. 203:2201.
2. Infantino S, et al. 2006. J. Immunol. 176:2197.

Regulation
Regulates the trafficking and activation of leukocytes
Gene ID
12778 View all products for this Gene ID 57007 View all products for this Gene ID
UniProt
View information about CXCR7 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 05.06.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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