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PE/Cyanine5 anti-mouse CD278 (ICOS) Antibody

Pricing & Availability
Clone
15F9 (See other available formats)
Regulatory Status
RUO
Other Names
Inducible COStimulatory molecule, H4, CD278
Isotype
Syrian Hamster IgG
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Product Citations
publications
15F9_PECy5_091907
ConA-stimulated C57BL/6 mouse splenocytes stained with 15F9 PE/Cyanine5
  • 15F9_PECy5_091907
    ConA-stimulated C57BL/6 mouse splenocytes stained with 15F9 PE/Cyanine5
Compare all formats See PE/Cyanine5 spectral data
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107708 100 µg 216€
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Description

CD278 is a 47-57 kD protein, also known as inducible costimulatory molecule (ICOS), or H4. This protein is homologous to the CD28/CTLA-4 proteins. It forms homodimers and is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. It has also been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Dong C, et al. 2001. Nature 409(6816):97.
  2. Walker LSK, et al. 2003. J. Immunol. 170:91.
Product Citations
  1. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  2. Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed
  3. Beus J, et al. 2013. PLoS One. 8:71221. PubMed
  4. Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed
  5. Jasti S, et al. 2017. Biol Reprod. 96:134. PubMed
  6. Jeljeli M, et al. 2021. Front Immunol. 12:670776. PubMed
  7. Wigren M, et al. 2009. Circ Res. 104:62. PubMed
RRID
AB_313337 (BioLegend Cat. No. 107708)
Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
CD28/CTLA-4, 47-57kD
Distribution

Activated T cells, subset of thymocytes

Function
Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor
B7h/B7RP-1/GL-50
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544.
  2. McAdam AJ, et al. 2000. J. Immunol. 165:5035.
  3. Mak TW, et al. 2003. Nat. Immunol. 4:765
Gene ID
54167 View all products for this Gene ID
UniProt
View information about CD278 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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