PE anti-rat RT1B Antibody

Pricing & Availability
Clone
OX-6 (See other available formats)
Regulatory Status
RUO
Other Names
MHC-class II, I-A equivalent
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
OX-6_PE_022309.jpg
LOU rat splenocytes stained with 1F4 (CD3) Alexa Fluor® 647 and OX-6 PE
  • OX-6_PE_022309.jpg
    LOU rat splenocytes stained with 1F4 (CD3) Alexa Fluor® 647 and OX-6 PE
See PE spectral data
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205308 100 µg 156,00€
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Description

RT1B is expressed on peripheral B lymphocytes, epidermal Langerhans cells, dendritic cells, some tissue macrophages, subsets of thymocytes, peritoneal mast cells, and small intestinal villus epithelium. The OX-6 antibody reacts with non-polymorphic determinants of the rat MHC class II antigen, I-A equivalent. The OX-6 monoclonal antibody cross-reacts with mouse I-A[k] and I-A[s] alloantigens and with a major subset of splenocytes from NOD (I-A [g7]) mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat, Mouse (I-A[k] and I-A[s] alloantigens and with a major subset of splenocytes from NOD (I-A [g7]) mice)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ia-like Glycoproteins from Wistar Thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.015 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Fox CC, et al. 1994. Cell Immunol. 158:253.
  2. Chen-Woan M, et al. 1996. J. Leukocyte Biol. 59:196.
  3. Damoiseaux JG, et al. 1998. J. Leukocyte Biol. 64:803.
  4. De Riva A, et al. 2013. J. Immunol. 190:5961. PubMed
Product Citations
  1. Wyatt‐Johnson SK et al. 2018. Frontiers in neurology. 0.819444444 . PubMed
  2. Louw A, et al. 2016. Nanomedicine. 12: 643-653. PubMed
RRID
AB_1595483 (BioLegend Cat. No. 205308)

Antigen Details

Structure
MHC-class II
Distribution

B lymphocytes, epidermal Langerhans cells, dendritic cells, some tissue macrophage, and subsets of thymocytes.

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
B cells, Dendritic cells, Langerhans cells, Macrophages, Thymocytes
Biology Area
Immunology
Molecular Family
MHC Antigens
Gene ID
24738 View all products for this Gene ID
UniProt
View information about RT1B on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12.14.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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