PE anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody

Pricing & Availability
Clone
OX-7 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1, Thy-1.1
Isotype
Mouse IgG1, κ
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Product Citations
publications
OX-7_PE_042909.jpg
LOU rat thymocytes stained with OX-7 PE
  • OX-7_PE_042909.jpg
    LOU rat thymocytes stained with OX-7 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
202523 50 µg 48,00€
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202524 200 µg 120,00€
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Description

CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, stromal cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat, Mouse (AKR/J, PL, and FVBIN mouse strains), Cross-Reactivity: Rabbit (Lapine), Guinea Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat thymocyte Thy-1 antigen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.

Additional reported applications (for the relevant formats) include: immunohistochemical7 and immunofluorescent8 staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation1, Western blotting1, in vitro activation of leukocytes2, induction of endothelial cell permeability3, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritis in vivo4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jeng CJ, et al. 1998. J. Cell Biol. 140:685. (IP, WB)
  2. Nakashima I, et al. 1991. J. Immunol. 147:1153.
  3. Ishizu A, et al. 1995. Int. Immunol. 7:1939.
  4. Eitner F. 1997. Kidney. Int. 51:69.
  5. Kawachi H, et al. 1992. Clin. Exp. Immunol. 88:399. (WB)
  6. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
  7. Daniel C, et al. 2012. Lab Invest. 92:812. (IHC-P)
  8. Li B, et al. 2006. Kidney Int. 69:323. (ICC)
  9. Uchimura H, et al. 2005. J Am Soc Nephrol. 16(4):997-1004. (IHC-F)
  10. Inagi R, et al. 2008. J Am Soc Nephrol. 19(5):915-22. (IHC-P)
Product Citations
  1. Paschalis E, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.02.005. PubMed
  2. Place D, et al. 2017. PLoS One. 10.1371/journal.pone.0190384. PubMed
  3. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  4. Wang D, et al. 2018. Immunity. 48:659. PubMed
  5. Hsu M, et al. 2019. Nat Commun. 10:229. PubMed
  6. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  7. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  8. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  9. Nurbaeva MK, et al. 2018. Front Physiol. 0.93125. PubMed
  10. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  11. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  12. Li C, et al. 2020. Immunity. 52(1):201-202. PubMed
  13. Alsughayyir J, et al. 2019. Front Immunol. 2.485416667. PubMed
  14. O’Connor R, et al. 2010. J Immunol. 185:7235. PubMed
  15. Olson M, et al. 2012. PLoS Pathog. 8:e1003054. PubMed
  16. Pauken K, et al. 2013. Diabetes. 62:2859. PubMed
  17. Cizkova D, et al. 2014. Sci Rep. 4:7514. PubMed
  18. Eriksson E, et al. 2016. Gene Ther. 10.1038/gt.2016.80. PubMed
  19. Campesato LF, et al. 2020. Nat Commun. 3.24375. PubMed
  20. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  21. Yan H, et al. 2020. Adv Sci (Weinh). 7:1903516. PubMed
  22. Chen M, et al. 2021. Bioact Mater. 1.591666667. PubMed
  23. Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed
  24. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
RRID
AB_1595635 (BioLegend Cat. No. 202523)
AB_1595524 (BioLegend Cat. No. 202524)

Antigen Details

Structure
Ig superfamily, GPI-anchored membrane glycoprotein, 28-30 kD
Distribution

Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli

Function
Adhesion, signal transduction, lymphocyte co-stimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor
Interacts with CD45
Cell Type
B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Mast cells, Neurons, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.

Gene ID
21838 View all products for this Gene ID 24832 View all products for this Gene ID
UniProt
View information about CD90/CD90.1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 08.06.2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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