PE anti-mouse RAE-1δ Antibody

Pricing & Availability
Clone
Charlotte d1.23 (See other available formats)
Other Names
Retinoid acid early inducible gene-1δ, RAE-1δ, RAE-1δ
Isotype
Mouse IgG1
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Product Citations
publications
ChD123_PE_042109.jpg
RAE-1δ transfected cells stained with Charlotte d1.23 PE
  • ChD123_PE_042109.jpg
    RAE-1δ transfected cells stained with Charlotte d1.23 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
133203 50 µg 80€
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Description

RAE-1δ is one of the five RAE-1 family, GPI-linked membrane protein consisting of alpha, beta, gamma, delta, and epsilon. They are strong homology within the family, related by 92%-95% sequence identity. They are distantly related to MHC class I proteins. RAE-1 proteins are abundantly expressed in fetal tissues, but not in normal adult tissue. They are constitutively expressed on some tumors and can be induced by retinoid acid. Its ligand NKG2D is found on NK cells, many transformed cell lines, and LPS-stimulated peritoneal macrophages. The interaction of NKG2D with RAE-1 transmits stimulatory signals to NK cells and other hematopoietic cells, leading to enhanced proliferation, cytokine secretion and target killing. They are involved in the regulation of innate and immune cytotoxic responses to tumors, pathogen-infected cells, and autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse RAE-1δ transfected RMA-S cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.015 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Lodoen M, et al. 2003. J. Exp. Med. 197:1245
  2. Carayannopoulos LN, et al. 2002. Eur J. Immunol.32 (3):597
RRID
AB_1595625 (BioLegend Cat. No. 133203)

Antigen Details

Structure
RAE is a family of GPI-linked membrane protein consisting of alpha, beta, gamma, delta, and epsilon. They are strong homology within the family, related by 92%-95% sequence identity. They are distantly related to MHC class I proteins.
Distribution
RAE-1 proteins are abundantly expressed in fetal tissues, but not in normal adult tissue. They are constitutively expressed on some tumors and can be induced by retinoid acid.
Ligand/Receptor
NKG2D
Biology Area
Immunology, Innate Immunity
Antigen References

1. Cerwenka A, et al. 2000. Immunity 12:721
2. Lodoen M, et al. 2003. J. Exp. Med. 197:1245
3. Diefenbach A, et al. 2001. Nature 413:165

Gene ID
66679 View all products for this Gene ID
UniProt
View information about RAE-1 delta on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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