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PE anti-mouse/human Ki-67 Antibody

Pricing & Availability
Clone
11F6 (See other available formats)
Regulatory Status
RUO
Other Names
Mki67, Ki67, Ki-67, MIB-1, KIA
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
11F6_PE_Ki-67_Antibody_091917
ConA/IL-2 treated C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol at -20°C for one hour, and then stained with 0.25 µg/T Ki-67 (clone 11F6) PE (filled histogram) or 0.25 µg/T rat IgG2b, κ PE isotype control (open histogram).
  • 11F6_PE_Ki-67_Antibody_091917
    ConA/IL-2 treated C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol at -20°C for one hour, and then stained with 0.25 µg/T Ki-67 (clone 11F6) PE (filled histogram) or 0.25 µg/T rat IgG2b, κ PE isotype control (open histogram).
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151209 25 µg 123€
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151210 100 µg 288€
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Description

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  2. Lin J, et al. 2021. Front Immunol. 12:796260. PubMed
  3. Low MSY, et al. 2019. Cell Reports. 29(9):2634-2645.e5. PubMed
  4. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  5. Hasmim M, et al. 2022. Front Immunol. 13:982821. PubMed
  6. Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed
  7. Zotos D, et al. 2021. Nat Commun. 12:7160. PubMed
  8. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
  9. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  10. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  11. Wang H, et al. 2019. Stem Cells Int. 2019:4686132. PubMed
  12. Shan L, et al. 2017. Immunity. 47:766. PubMed
RRID
AB_2716014 (BioLegend Cat. No. 151209)
AB_2716008 (BioLegend Cat. No. 151210)

Antigen Details

Structure
325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
Distribution

Nucleus and chromosomes.

Function
Required for cell cycle progression and proliferation.
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References
  1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
  2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
  3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
  4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
  5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
  6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID
4288 View all products for this Gene ID 17345 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 09.19.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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