PE anti-mouse CD282 (TLR2) Antibody

Pricing & Availability
Clone
CB225 (See other available formats)
Other Names
TLR-2, mTLR2, Ly105
Isotype
Rat IgG2a, κ
Ave. Rating
1 reviews
Product Citations
publications
CB225_TLR2_PE_Antibody_FC_072814.jpg
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with TLR2 (clone CB225) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • CB225_TLR2_PE_Antibody_FC_072814.jpg
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with TLR2 (clone CB225) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Quantity Avail. Save
148603 25 µg 92€
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148604 100 µg 228€
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Description

CD282, also known as TLR2, is an 89 kD, type I transmembrane protein, with 14 leucine-rich repeats (LRR) in the extracellular region and one toll/IL-1R (TIR) domain in the intracellular region. TLR2 is mainly expressed on monocytes, macrophages, dendritic cells, granulocytes, and endothelial and epithelial cells. TLR2 associates with TLR1 and TLR6, and mediates the innate immune response against several components of Gram-positive bacteria, such as peptidoglycan, lipoteichoic acid (LTA), lipoarabinomanan, and lipoproteins as well as different LPS from certain Gram-negative bacteria, yeast, spirochetes, and fungi.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TLR2-transfected Ba/F3 cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

1. Shibata T, et al. 2012. Int. Immunol. 24:613. (FC)

Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Lax N, et al. 2020. J Neuroinflammation. 17:55. PubMed
RRID
AB_2564119 (BioLegend Cat. No. 148603)
AB_2564120 (BioLegend Cat. No. 148604)

Antigen Details

Structure
89 kD type I transmembrane protein with 14 leucine-rich repeats (LRR) on its extracellular region and one toll/IL-1R (TIR) domain on its intracellular region.
Distribution

Monocytes, macrophages, dendritic cells, granulocytes, endothelial cells, epithelial cells.

Function
Activates innate host defense against a diverse set of pathogens.
Interaction
TLR1, TLR6.
Ligand/Receptor
Microbial lipoproteins, peptidoglycans, lipoteichoic acid.
Cell Type
Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Du B, et al. 2013. J. Biol. Chem. 288:15131.
2. Zivkovic A, et al. 2011. J. Immunol. 186:1608. 
3. Okun E, et al. 2010. J. Neurochem. 114:462. 
4. Kim S, et al. 2009. Nature. 457:102.

Gene ID
24088 View all products for this Gene ID
UniProt
View information about CD282 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 06.27.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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