PE anti-mouse CD150 (SLAM) Antibody

Pricing & Availability
Clone
TC15-12F12.2 (See other available formats)
Regulatory Status
RUO
Other Names
Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
Isotype
Rat IgG2a, λ
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Product Citations
publications
TC15-12F12.2
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12.2
    C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12_PE_2_102110
    C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE (filled histogram) or rat IgG2a PE isotype control (open histogram) (gated on lymphoid cell population).
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115903 25 µg 76€
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115904 100 µg 194€
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Description

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse SLAM-human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD150 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).

Application References

(PubMed link indicates BioLegend citation)
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  2. Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
  3. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
  4. Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
  5. Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
  6. Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
  7. Jung Y, et al. 2007. Blood 110:82. PubMed
  8. Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
  9. Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
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  14. Song J, et al. 2010. Blood 115:2592. PubMed
  15. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
  16. Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
  17. Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
Product Citations
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  15. Ley C, et al. 2015. Cancer Cell. 27: 631-43. PubMed
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  18. Kokkaliaris K, et al. 2016. Blood. 128: 1181 - 1192. PubMed
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  55. Ise W, et al. 2018. Immunity. 48:702. PubMed
  56. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  57. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  58. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
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  77. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  78. Kim I, et al. 2006. Blood. 108:737. PubMed
  79. Jung Y, et al. 2007. Blood . 110:82. PubMed
  80. Jordan M, et al. 2007. J Immunol. 178:1618. PubMed
  81. Fraser S, et al. 2007. Blood. 109:343. PubMed
  82. Jung Y, et al. 2008. Stem Cells. 26:2042. PubMed
  83. Morita Y, et al. 2010. J Exp Med. 207:1173. PubMed
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RRID
AB_313682 (BioLegend Cat. No. 115903)
AB_313683 (BioLegend Cat. No. 115904)

Antigen Details

Structure
Ig superfamily, 75-95 kD
Distribution

Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells

Function
B cell and dendritic cell costimulation
Ligand/Receptor
CD150
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.

Gene ID
27218 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD150 (SLAM)

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  • Biotin anti-mouse CD150 (SLAM)

  • APC anti-mouse CD150 (SLAM)

  • PE/Cyanine5 anti-mouse CD150 (SLAM)

  • PE/Cyanine7 anti-mouse CD150 (SLAM)

  • Alexa Fluor® 488 anti-mouse CD150 (SLAM)

  • Alexa Fluor® 647 anti-mouse CD150 (SLAM)

  • PerCP/Cyanine5.5 anti-mouse CD150 (SLAM)

  • Pacific Blue™ anti-mouse CD150 (SLAM)

  • Brilliant Violet 421™ anti-mouse CD150 (SLAM)

  • Brilliant Violet 605™ anti-mouse CD150 (SLAM)

  • Brilliant Violet 510™ anti-mouse CD150 (SLAM)

  • Brilliant Violet 650™ anti-mouse CD150 (SLAM)

  • Purified anti-mouse CD150 (SLAM) (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-mouse CD150 (SLAM)

  • Brilliant Violet 785™ anti-mouse CD150 (SLAM)

  • APC/Fire™ 750 anti-mouse CD150 (SLAM)

  • Brilliant Violet 711™ anti-mouse CD150 (SLAM)

  • TotalSeq™-A0203 anti-mouse CD150 (SLAM)

  • TotalSeq™-C0203 anti-mouse CD150 (SLAM)

  • Ultra-LEAF™ Purified anti-mouse CD150 (SLAM)

  • TotalSeq™-B0203 anti-mouse CD150 (SLAM)

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