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PE anti-mouse CD125 (IL-5Rα) Antibody

Pricing & Availability
Clone
DIH37 (See other available formats)
Regulatory Status
RUO
Other Names
IL-5Rα, IL-5 Receptor α subunity
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
DIH37_PE_CD125_Antibody_1_090617
Mouse peripheral blood granulocytes were stained with CD193 PE/Cy7 and CD125 (clone DIH37) PE (top) or rat IgG2a, κ (clone RTK2758) PE isotype control (bottom)
  • DIH37_PE_CD125_Antibody_1_090617
    Mouse peripheral blood granulocytes were stained with CD193 PE/Cy7 and CD125 (clone DIH37) PE (top) or rat IgG2a, κ (clone RTK2758) PE isotype control (bottom)
  • DIH37_PE_CD125_Antibody_2_090617
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153403 25 µg 123€
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153404 100 µg 268€
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Description

CD125 is a 60 kD type I transmembrane protein. It is the ligand-specific α subunit of the heterodimeric interleukin 5 (IL-5) receptor. The signal-transducing β subunit of this receptor does not bind to any cytokines and is shared by the receptors for IL-3 and CSF2/GM-CSF. IL-5 is mainly produced by Th2 cells and elevated IL-5 levels are associated with allergic disorders. CD125 has also been found to interact with syndecan binding protein (SDCBP or syntenin), leading to the activation of the transcription factor SOX4. The mouse CD125 is expressed on eosinophils, basophils, and B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine IL-5Ralpha-hFcgamma1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Zhang Q, et al. 2022. Commun Biol. 5:544. PubMed
RRID
AB_2715828 (BioLegend Cat. No. 153403)
AB_2715829 (BioLegend Cat. No. 153404)

Antigen Details

Structure
60 kD type I transmembrane protein
Distribution

Eosinophils, basophils and B cells

Function
IL-5 receptor
Interaction
JAK2, CD131 (IL3RB, CSF2RB), SDCBP
Ligand/Receptor
IL-5
Cell Type
B cells, Basophils, Eosinophils
Biology Area
Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Takaki S, et al. 1990. EMBO J. 9:4367
2. Takatsu K, et al. 2011. Proc. Jpn. Acad. Ser B. 87:463.
3. Hitoshi Y, et al. 1990. J. Immunol. 144:4218.

Gene ID
16192 View all products for this Gene ID
UniProt
View information about CD125 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 09.06.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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