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PE anti-mouse 4-1BB Ligand (CD137L) Antibody

Pricing & Availability
Clone
TKS-1 (See other available formats)
Regulatory Status
RUO
Other Names
4-1BBL, CD137L, TNFSF9, 4-1BB Ligand
Isotype
Rat IgG2a, κ
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Product Citations
publications
TKS-1_PE_072407
Mouse B cell line 2PK3 stained with TKS-1 PE
  • TKS-1_PE_072407
    Mouse B cell line 2PK3 stained with TKS-1 PE
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107105 50 µg 88€
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Description

4-1BB ligand, also known as CD137L, is a 97 kD member of the TNF superfamily present on antigen-presenting cells (dendritic cells and macrophages) and activated B and T lymphocytes. 4-1BBL interacts with 4-1BB (CD137) to induce T and B cell costimulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse B lymphoma line 2PK-3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TKS-1 antibody inhibits binding of soluble 4-1BB to 4-1BBL in vitro. Additional reported applications (for the relevant formats) include: in vitro blocking the binding of 4-1BB to 4-1BBL1. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107106) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107104) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 107108).

Application References

(PubMed link indicates BioLegend citation)
  1. Akiba H, et al. 2000. J. Exp. Med. 191:375. (Block)
  2. Futagawa T, et al. 2002. Int. Immunol. 14:275.
  3. Nishimoto H, et al. 2005. Blood 106:4241. (FC) PubMed
  4. De Keersmaecker B, et al. 2011. J Leukoc Biol. 89:989. PubMed.
  5. Zhao Y, et al. 2012. Immuno Lett. 141:220. PubMed.
Product Citations
  1. Martin S, et al. 2016. PLoS One. 11: 0155189. PubMed
  2. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  3. Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed
  4. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  5. Laspidea V, et al. 2022. JCI Insight. 7:. PubMed
  6. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  7. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  8. Nishimoto H, et al. 2005. Blood. 106:4241. PubMed
RRID
AB_2256408 (BioLegend Cat. No. 107105)

Antigen Details

Structure
NGF/TNF superfamily, 97 kD
Distribution

Activated B cells and T cells, macrophages, and dendritic cells

Function
T cell costimulation
Ligand/Receptor
4-1BB (CDw137)
Cell Type
B cells, Dendritic cells, Macrophages, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Pollok KE, et al. 1995. Eur. J. Immunol. 25:488.
2. DeBenedette MA, et al. 1997. J. Immunol. 158:551.
3. Pollok KE, et al. 1993. J. Immunol. 150:771.
4. Pollok KE, et al. 1994. Eur. J. Immunol. 24:367.
5. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.

Gene ID
21942 View all products for this Gene ID
UniProt
View information about CD137L on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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