Typical result of PMA + Ionomycin with Brefeldin A stimulated (4-hour) human peripheral blood lymphocytes fixed with Fixation Buffer (Cat# 420801), permeabilized with Permeabilization Wash Buffer (Cat# 421002), and intracellularly stained either with MP4-25D2 PE used at 5 µL/test (left) or with an isotype control (right).
Typical result of PMA + Ionomycin with Brefeldin A stimulated (4-hour) human peripheral blood lymphocytes fixed with Fixation Buffer (Cat# 420801), permeabilized with Permeabilization Wash Buffer (Cat# 421002), and intracellularly stained either with MP4-25D2 PE used at 5 µL/test (left) or with an isotype control (right).
IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.
Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
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Avoid prolonged exposure of the reagent or stained cells to light.
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