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PE anti-human IL-3 Antibody

Pricing & Availability
Clone
BVD3-1F9 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
Isotype
Rat IgG1, κ
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Product Citations
publications
BVD3-1F9_PE_030408
Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then stained with CD4 PE/Cy5 and BVD3-1F9 PE
  • BVD3-1F9_PE_030408
    Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then stained with CD4 PE/Cy5 and BVD3-1F9 PE
See PE spectral data
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500606 100 tests 244€
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Description

IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD3-1F9 antibody can neutralize the bioactivity of natural or recombinant IL-3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Yeast-expressed, recombinant human IL-3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA1-3,6 or ELISPOT4 Detection: The biotinylated BVD3-1F9 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified BVD8-3G11 (Cat. No. 500502) antibody as the capture antibody.
Flow Cytometry: The fluorochrome-labelled BVD3-1F9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-3 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Additional reported applications (for the relevant formats) include: immunoprecipitation6, Western blotting6, neutralization1,6, immunohistochemical staining5,7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunological Reviews 127:5.
  2. Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133.
  3. Abrams J. 1995. Curr. Prot. Immunol.. 6.20.
  4. Mahanty S, et al. 1992. J. Immunol. 148:3567.
  5. Andersson U, et al. 1993. Detection and quantification of gene expression. New York: Springer-Verlag.
  6. Kaushansky K. 1992. J. Clin. Invest. 90:1879.
  7. Andersson J, et al. 1994. Immunology 83:16.
RRID
AB_2123712 (BioLegend Cat. No. 500606)

Antigen Details

Structure
Cytokine; 14-30 kD (Mammalian)
Bioactivity
Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase
Cell Sources
Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells
Cell Targets
Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
Receptors
IL-3R, α subunit (CD123), common β subunit (CDw131)
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Frendl G. 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle J. 1992. Chem. Immunology 51:65.

Regulation
Upregulated by T cell stimulation; downregulated by glucocorticoids
Gene ID
3562 View all products for this Gene ID
UniProt
View information about IL-3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All IL-3 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-human IL-3 BVD3-1F9 ELISA Detection,ELISPOT Detection
PE anti-human IL-3 BVD3-1F9 ICFC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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