CD51/CD61 is an integrin complex known as αVβ3. It is expressed at high levels on osteoclasts, endothelial cells, and melanoma cells and at low levels on platelets and macrophages. CD51 is a heterodimer composed of disulfide-linked 125 kD and 24 kD proteins. CD61 is also a member of the integrin family known as gpIIIa or β3 integrin. It is a 110 kD common β subunit of CD51/CD61 or CD41/CD61 complex. CD51/CD61, also known as the vitronectin receptor, mediates the binding of platelets to immobilized vitronectin without prior activation. Other ligands include RGD-containing proteins such as fibrinogen, fibronectin, von Willebrand factor (vWf), laminin, thrombospondin and the neural adhesion molecule L1. CD51/CD61 also mediates cell-cell adhesion via interaction with CD31. CD51/CD61 acts as an activation-independent receptor for platelet attachment and spreading on vitronectin and other RGD-containing proteins, including matrix components. The 23C6 antibody has been reported to be useful for blocking studies.
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation5, immunohistochemical staining of acetone-fixed frozen tissue sections5, immunofluorescence microscopy5, and blocking of cell adhesion4,6. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 304414).
Application References
(PubMed link indicates BioLegend citation)
Knapp WB, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Horton M, et al. 1991. Exp. Cell Res. 195:368.
Takahashi R, et al. 1999. Blood 93:1951. (Block)
Davies J, et al. 1989. J. Cell Biol. 109:1817. (IF, IHC, IP)
Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709. (FC, Block)
Barau A, et al. 2010. J. Ultrasound Med. 29:173. PubMed
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