PE anti-human CD300F (IREM-1) Antibody

Pricing & Availability
Clone
UP-D2 (See other available formats)
Regulatory Status
RUO
Other Names
CD300LF, IREM-1, IgSF13,
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
UP-D2_PE_062509.jpg
Human peripheral blood monocytes stained with UP-D2 PE
  • UP-D2_PE_062509.jpg
    Human peripheral blood monocytes stained with UP-D2 PE
See PE spectral data
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340604 100 tests 236,00€
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Description

IREM-1 (immune receptor expressed by myeloid cell 1) is a type I single transmembrane protein encoded by human chromosome 17q24.25. A member of the CD300 (CMRF-35) family and the Ig superfamily, IREM-1 is also known as CD300F, or CD300LF (CD300-like member F). CD300F is expressed on monocytes, granulocytes, and myeloid leukemia cell lines. The cytoplasmic domain of IREM-1 contains an ITIM and associates with the P85α subunit of PI3K (phosphoinositide 3-kinase). IREM-1 also functions as an inhibitory receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: Coengagement of IREM-1 with UP-D2 inhibits FcReI-mediated degranulation, immunoprecipitation

Application References

(PubMed link indicates BioLegend citation)
  1. Alvarez-Errico D, et al. 2004. Eur. J. Immunol. 34:3690
  2. Lee SM, et al. 2011. J. Immunol. 186:6296. PubMed.
RRID
AB_2074121 (BioLegend Cat. No. 340604)

Antigen Details

Structure
Type I single transmembrane protein with cytoplamic ITIM, associates with p85α of PI3K, Ig superfamily
Distribution

Monocytes, granulocytes, and myeloid leukemia cell lines

Function
Inhibition of myeloid function
Cell Type
Monocytes, Granulocytes, Leukemia
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Marquez JA, et al. 2007. J. Mol. Bio. 367:310
2. Alvarez-Errico D, et al. 2004. Eur. J. Immunol. 34:3690
3. Alvarez-Errico D, et al. 2007. J. Immunol. 178:808

Gene ID
146722 View all products for this Gene ID
UniProt
View information about CD300F on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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