PE anti-human CD3 Antibody

Pricing & Availability
Clone
HIT3a (See other available formats)
Regulatory Status
RUO
Workshop
V CD03.05
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HIT3a
Human peripheral blood lymphocytes stained with HIT3a PE
  • HIT3a
    Human peripheral blood lymphocytes stained with HIT3a PE
See PE spectral data
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300307 25 tests 20,00€
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300308 100 tests 44,00€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
  8. Enghard P, et al. 2014. Ann Rheum Dis. 73:277. PubMed
Product Citations
  1. Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed
  2. Lin GL, et al. 2018. Acta Neuropathol Commun. 9:617. PubMed
  3. Rochat MA, et al. 2018. Virol J. 15:191. PubMed
  4. Blandine Monel et al. 2019. Cell reports. 27(1):142-153 . PubMed
  5. Wei J, et al. 2019. J Immunother Cancer. 7:209. PubMed
  6. Usmani SM et al. 2019. Cell host & microbe. 25(1):73-86 . PubMed
  7. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  8. Mujib S, et al. 2017. JCI Insight. 2:e93687. PubMed
  9. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  10. Hu JQ, et al. 2020. J Cancer. 3.409722222. PubMed
  11. Łyszkiewicz M, et al. 2020. Nat Commun. 11:1031. PubMed
  12. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  13. Wang H, et al. 2020. J Exp Clin Cancer Res. 39:29. PubMed
  14. Bardet M, et al. 2018. Immunol Cell Biol. 96:81. PubMed
  15. Liu F, et al. 2020. Oncoimmunology. 9:1746573. PubMed
  16. Zhai Y, et al. 2018. Autophagy. 1.714583333. PubMed
  17. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  18. Wu W, et al. 2020. Oncol Lett. 0.91875. PubMed
  19. Enghard P, et al. 2014. Ann Rheum Dis. 73:277. PubMed
  20. Galindo-Albarrán A, et al. 2016. Cell Rep. 17:2151-2160. PubMed
  21. Park JA, et al. 2020. J Hematol Oncol. 0.661111111. PubMed
  22. Park JA, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
  23. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  24. Mori A, et al. 2021. Cells. :10. PubMed
  25. Wu M, et al. 2021. Nat Commun. 12:3500. PubMed
RRID
AB_314043 (BioLegend Cat. No. 300307)
AB_314044 (BioLegend Cat. No. 300308)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK-T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
T cells, NKT cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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