- Basophil/2D7 (See other available formats)
- Mouse IgG1, κ
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The Basophil/2D7 mAb specifically recognizes the human basophil population. The antigen that this antibody binds is present in cell granules but its identity is still unknown.Product Details
- Antibody Type
- Host Species
- Lysates of purified basophils
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications for the relevant formats include: immunofluorescence staining7, and immunohistochemistry of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1-6.
(PubMed link indicates BioLegend citation)
- Kepley CL, et al. 1995. J. Immunol. 154:6548. (IHC)
- Irani AM, et al. 1998. J. Allergy Clin. Immunol. 1998 101:354. (IHC)
- Agis H, et al. 2006. J. Clin. Pathol. 59:396. (IHC)
- Plager DA, et al. 2006. J. Allergy Clin. Immunol. 117:626. (IHC)
- Sperr WR, et al. 2001. Blood 98:2200. (IHC)
- Aichberger KJ, et al. 2006. Blood 108:3538. (IHC)
- Kepley CL, et al. 1998. J. Allergy Clin. Immun. 102:304. (IF)
AB_11125171 (BioLegend Cat. No. 346206)
- Cell Type
- Biology Area
- Immunology, Innate Immunity
- Gene ID
- View information about Basophil on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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