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PE anti-BACH2 Antibody

Pricing & Availability
Clone
16B10B53 (See other available formats)
Regulatory Status
RUO
Other Names
BTB and CNC homolog 2, Transcription regulator protein BACH2,
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
16B10B53_PE_BACH2_Antibody_041522
Ramos cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with anti-BACH2 (clone 16B10B53) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 16B10B53_PE_BACH2_Antibody_041522
    Ramos cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with anti-BACH2 (clone 16B10B53) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
See PE spectral data
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695603 25 tests 172€
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695604 100 tests 336€
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Description

BTB and CNC Homolog 2 (BACH2) is a transcription factor of the basic leucine zipper family. BACH2 participates in the regulation of both innate and adaptive immune responses. In B cells, BACH2 plays critical roles in somatic hypermutation, class-switch recombination, memory B cell formation, and repression of myeloid genes. In T cells, BACH2 promotes the development of Foxp3+ regulatory T cells and memory T cells. Bach2 is required for the maintenance of naive CD4 T cells by suppressing effector T cell differentiation. BACH2 is also required for the maturation and function of alveolar macrophages. Defects in BACH2 gene are associated with autoimmune and allergic diseases, such as asthma, type 1 diabetes, celiac disease, rheumatoid arthritis, and generalized vitiligo.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human BACH2 recombinant protein (513-841 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2922645 (BioLegend Cat. No. 695603)
AB_2922645 (BioLegend Cat. No. 695604)

Antigen Details

Structure
841 amino acids with a predicted molecular weight of 92.5 kD. Contains a N-terminal BTB/POZ domain for protein-protein interaction and a C-terminal bZIP domain for DNA binding.
Distribution

Nucleus and cytoplasm.

Function
BACH2 is a transcription factor involved in the development and differentiation of both B and T cells.
Interaction
Forms homodimers or heterodimers with Maf-related transcription factors.
Cell Type
B cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Kuwahara M, et al. 2016. Nat. Commun. 7:12596.
2. Sidwell T, et al. 2016. Nat. Immunol. 17:744.
3. Roychoudhuri R, et al. 2016. Nat. Immunol. 17:851.
4. Itoh-Nakadai A, et al. 2014. Nat. Immunol. 15:1171.
5. Kuwahara M, et al. 2014. Nat. Commun. 5:3555.
6. Kim EH, et al. 2014. J. Immunol. 192:985.

Gene ID
60468 View all products for this Gene ID
UniProt
View information about BACH2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 04.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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