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Pacific Blue™ anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_Blue_080709.jpg
C57BL/6 mouse bone marrow cells stained with HK1.4 Pacific Blue™
  • HK1dot4_Blue_080709.jpg
    C57BL/6 mouse bone marrow cells stained with HK1.4 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
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128013 25 µg 100€
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128014 100 µg 212€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Orberg E, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.53. PubMed
  2. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  3. Frieler RA, et al. 2017. Exp Neurol. 298:104. PubMed
  4. Mathur R, et al. 2019. Mucosal Immunol. 12:612. PubMed
  5. Yang C, et al. 2022. Nat Commun. 13:3426. PubMed
  6. Zhang R, et al. 2019. Cell Rep. 28:2647. PubMed
  7. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  8. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
  9. Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed
  10. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  11. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  12. Lebratti T, et al. 2021. eLife. 10:00. PubMed
  13. Spees A, et al. 2014. Infect Immun . 82:1692. PubMed
  14. Bourn JR, et al. 2021. Cancer Lett. 503:75. PubMed
  15. Mooney J, et al. 2015. Sci Rep. 5: 14603. PubMed
  16. Li J, et al. 2019. JCI Insight. 5. PubMed
  17. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  18. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  19. Hoffmann J, et al. 2014. PLoS One. 9:98456. PubMed
  20. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  21. Jin C, et al. 2019. Cell. 176:998. PubMed
  22. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  23. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  24. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  25. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  26. Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
  27. Cunha LD et al. 2018. Cell. 175(2):429-441 . PubMed
  28. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  29. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  30. Li S, et al. 2019. Front Neurol. 1.036805556. PubMed
  31. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  32. Muchenditsi A, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 313:G39. PubMed
  33. Sadhu S, et al. 2021. J Immunol. 207:1812. PubMed
  34. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  35. Gharib S, et al. 2014. J Leukoc Biol. 95:9. PubMed
  36. Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
  37. Manríquez V, et al. 2021. Nat Commun. 12:4547. PubMed
  38. Kusnadi A, et al. 2020. Immunity. 51(2):241-257. PubMed
  39. Ho A, et al. 2011. J Immunol. 187:6011. PubMed
  40. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
RRID
AB_1732090 (BioLegend Cat. No. 128013)
AB_1732079 (BioLegend Cat. No. 128014)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12.09.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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