- 53-7.3 (See other available formats)
- Regulatory Status
- Other Names
- Lyt-1, Ly-1, T1, Tp67, Ly-12
- Rat IgG2a, κ
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- Product Citations
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CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse thymus or spleen
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.
(PubMed link indicates BioLegend citation)
- Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
- Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC)
- Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
AB_2813915 (BioLegend Cat. No. 100641)
AB_2813916 (BioLegend Cat. No. 100642)
- Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
Thymocytes, T cells, B-1 cells
- Negative regulator of T-B cell interaction
- CD72, gp35-37
- Cell Type
- B cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kipps TJ. 1988. Adv. Immunol. 47:117.
3. Antin JH, et al. 1985. J. Immunol. 136:505.
4. Tarakhovsky A, et al. 1995. Science 269:535.
- Gene ID
- 12507 View all products for this Gene ID
- View information about CD5 on UniProt.org
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FITC anti-mouse CD5
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Brilliant Violet 510™ anti-mouse CD5
Alexa Fluor® 488 anti-mouse CD5
Alexa Fluor® 647 anti-mouse CD5
PerCP anti-mouse CD5
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PE/Cyanine7 anti-mouse CD5
Purified anti-mouse CD5 (Maxpar® Ready)
PerCP/Cyanine5.5 anti-mouse CD5
APC anti-mouse CD5
Alexa Fluor® 594 anti-mouse CD5
APC/Fire™ 750 anti-mouse CD5
Alexa Fluor® 700 anti-mouse CD5
TotalSeq™-A0111 anti-mouse CD5
Brilliant Violet 711™ anti-mouse CD5
Pacific Blue™ anti-mouse CD5
PE/Dazzle™ 594 anti-mouse CD5
TotalSeq™-B0111 anti-mouse CD5
TotalSeq™-C0111 anti-mouse CD5
APC/Cyanine7 anti-mouse CD5