Pacific Blue™ anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
30-F11_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ 30-F11
  • 30-F11_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ 30-F11
See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
103125 25 µg 68,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103126 100 µg 156,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
  1. Choudhary S, et al. 2012. J Virol. 86:114. PubMed
  2. Perveen S, et al. 2012. PLoS One. 7:e51945. PubMed
  3. Wan W, et al. 2013. Cardiovasc Res. 97:580. PubMed
  4. Sungur C, et al. 2013. Proc Natl Acad Sci U S A. 110:7401. PubMed
  5. Lee C, et al. 2014. Cancer Res. 74:341. PubMed
  6. Chan A, et al. 2014. Am J Pathol. 184:1411. PubMed
  7. Shade K, et al. 2015. J Exp Med. 212:457. PubMed
  8. Cheah M, et al. 2015. Proc Natl Acad Sci U S A. 112:4725. PubMed
  9. Oliveira-Lima OC, et al. 2015. Immunobiology. . PubMed
  10. McCoy S 2015. Am J Physiol Lung Cell Mol Physiol. 309: L463-L474. PubMed
  11. Rosen D, et al. 2016. J Infect Dis. 213: 649 - 658. PubMed
  12. Pattabiraman D, et al. 2016. Science. 351: aad3680. PubMed
  13. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  14. Chang J, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2015121356. PubMed
  15. Nie M, et al. 2016. Cell Death Dis. 7:e2261. PubMed
  16. Skowron K, et al. 2016. Sci Rep. 6:35854. PubMed
  17. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  18. Scheele C, et al. 2017. Nature. 542:313-317. PubMed
  19. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  20. Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed
  21. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
  22. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  23. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  24. Barsoumian HB, et al. 2020. J Immunother Cancer. 8:00. PubMed
  25. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  26. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  27. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  28. Cortez MA, et al. 2020. Nat Commun. 3.819444444. PubMed
  29. Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.347916667. PubMed
  30. Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed
  31. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  32. Yao Z, et al. 2020. Cancer Res. 80:1171. PubMed
  33. Hemanthakumar KA, et al. 2021. eLife. 10:00. PubMed
  34. Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed
  35. Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed
  36. Huang S, et al. 2020. Cell. 184(2):441-459.e25. PubMed
  37. Stahnke S, et al. 2021. Current Biology. 31(10):2051-2064.e8. PubMed
  38. Chen Y, et al. 2021. Cancer Cell. 39(4):548-565.e6. PubMed
  39. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  40. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  41. Barkaway A, et al. 2021. Immunity. . PubMed
  42. Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
  43. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  44. Peuhu E, et al. 2017. PLoS One. 12:e0186628. PubMed
  45. Eilken H, et al. 2017. Nat Commun. . 10.1038/s41467-017-01738-3. PubMed
  46. Kato K, et al. 2018. Nat Commun. 48:120. PubMed
  47. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  48. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  49. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  50. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  51. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  52. Tucey TM et al. 2018. Cell metabolism. 27(5):988-1006 . PubMed
  53. Avey D et al. 2018. Cell reports. 24(13):3619-3629 . PubMed
  54. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
  55. Girbl T et al. 2018. Immunity. 49(6):1062-1076 . PubMed
  56. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  57. Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed
  58. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  59. Bignami F, et al. 2017. Acta Ophthalmol. 95:e641. PubMed
  60. Engblom C, et al. 2017. Science. 358:6367. PubMed
  61. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
  62. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  63. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  64. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  65. Li C, et al. 2018. Cell. 174:285. PubMed
  66. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  67. Iwata TN, et al. 2018. Mol Cancer Ther. 17:1494. PubMed
  68. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  69. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  70. Diéguez–Hurtado R, et al. 2019. Nat Commun. 10:2817. PubMed
  71. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
  72. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  73. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  74. Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed
  75. McKenzie MD, et al. 2019. Cell Stem Cell. 25:258. PubMed
  76. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  77. Zilionis R et al. 2019. Immunity. 50(5):1317-1334 . PubMed
  78. Forese MG et al. 2019. Glia. 68(1):95-110 . PubMed
  79. Gomes AM, et al. 2018. Cell Rep. 25:2821. PubMed
  80. Li Y et al. 2018. Cell Stem Cell. 23(2):181-192 . PubMed
  81. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  82. Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed
  83. Lee S et al. 2017. Cell host & microbe. 22(4):449-459 . PubMed
  84. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  85. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  86. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  87. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  88. Chia JSJ, et al. 2018. Nutrients. 1.313194444. PubMed
  89. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  90. Barry DM, et al. 2019. Nat Commun. 4.378472222. PubMed
  91. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  92. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  93. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  94. Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
  95. Maas SLN, et al. 2020. J Neuroinflammation. 17:120. PubMed
  96. Frasca D, et al. 2020. Front Immunol. 11:328. PubMed
  97. Wietecha MS, et al. 2020. Nat Commun. 2.266666667. PubMed
  98. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  99. Caetano MS, et al. 2019. Clin Cancer Res. 25:7576. PubMed
  100. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  101. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  102. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  103. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  104. Fernandes RA, et al. 2020. eLife. 9:e58463.. PubMed
  105. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  106. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  107. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  108. Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
  109. Jiang Q, et al. 2008. Blood. 112:2858. PubMed
  110. Ma L, et al. 2009. J Immunol. 183:1044. PubMed
  111. Shi C, et al. 2010. J Immunol. 184:6266. PubMed
  112. Carson W, et al. 2011. PLoS One. 6:e20385. PubMed
  113. Nishida C, et al. 2012. Blood. 119:5405. PubMed
RRID
AB_493536 (BioLegend Cat. No. 103125)
AB_493535 (BioLegend Cat. No. 103126)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05.14.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account