Pacific Blue™ anti-human Granzyme A Antibody

Pricing & Availability
CB9 (See other available formats)
Regulatory Status
Other Names
Granzyme 1, Hanukah factor serine protease (HFSP), Cytotoxic T-lymphocyte-associated serine esterase 3 (CTLA-3)
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes intracellularly stained with CD8 (RPA-T8) PerCP and CB9 Pacific Blue™
  • CB9_Blue_071108.jpg
    Human peripheral blood lymphocytes intracellularly stained with CD8 (RPA-T8) PerCP and CB9 Pacific Blue™
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507208 25 µg 90€
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507207 100 µg 212€
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Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Purified human Granzyme A
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2.

Application References

(PubMed link indicates BioLegend citation)
  1. Trimble L, et al. 1998. Blood 91:585.
  2. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285.
  3. Raqib R, et al. 2002. Infect. Immun. 70:3199.
  4. Chen H, et al. 2005. J. Immunol. 175:591.
Product Citations
  1. Dimova T, et al. 2015. Proc Natl Acad Sci U S A. 112:556. PubMed
  2. Kiniry BE, et al. 2018. J Immunol. 200:1876. PubMed
  3. Yamamoto T, et al. 2012. J Virol. 86:5877. PubMed
  4. Sanchez-Ruiz Y, et al. 2011. PLoS One. 6:e27057. PubMed
  5. Marquardt N, et al. 2015. J Immunol. 194:2467. PubMed
  6. Cellerai C, et al. 2010. J Virol. 84:3868. PubMed
AB_492996 (BioLegend Cat. No. 507208)
AB_439755 (BioLegend Cat. No. 507207)

Antigen Details

Serine protease; disulfide-linked homodimer; 28 kD (Mammalian)
Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking
Cell Sources
Cytotoxic T cells, NK cells
Cell Targets
Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1
Assisted by perforin
Cell Type
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Brune J, et al. 1986. Nature 322:268.
2. Fan Z, et al. 2003. Nature Immunol. 4:145.
3. Fan Z, et al. 2003. Cell 112:659.
4. Masson D, et al. 1987. Cell 49:679.

Gene ID
3001 View all products for this Gene ID
View information about Granzyme A on

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Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human Granzyme A

  • PE anti-human Granzyme A

  • Purified anti-human Granzyme A

  • Pacific Blue™ anti-human Granzyme A

  • Alexa Fluor® 700 anti-human Granzyme A

  • Alexa Fluor® 488 anti-human Granzyme A

  • Alexa Fluor® 647 anti-human Granzyme A

  • PerCP/Cyanine5.5 anti-human Granzyme A

  • Alexa Fluor® 594 anti-human Granzyme A

  • APC anti-human Granzyme A

  • PE/Cyanine7 anti-human Granzyme A

  • TotalSeq™-B1099 anti-human Granzyme A


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