MojoSort™ Mouse CD11b Selection Kit

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Other Names
Mouse CD11b Positive Selection, CD11b Positive selection
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MojoSort_Mouse_CD11b_Selection_Kit_1_082820
A single cell suspension from C57BL/6 mouse bone marrow was prepared for the positive selection of CD11b+ cells using the MojoSort™ Mouse CD11b+ Selection Kit. Left plot: CD11b+ cells after isolation. Right plot: Cells before isolation. Cells were stained with anti-mouse CD11b+ (clone M1/70) APC, Ly-6G/Ly-6C (Gr-1) PE. Dead cells were excluded by Helix NP™ Blue.
  • MojoSort_Mouse_CD11b_Selection_Kit_1_082820
    A single cell suspension from C57BL/6 mouse bone marrow was prepared for the positive selection of CD11b+ cells using the MojoSort™ Mouse CD11b+ Selection Kit. Left plot: CD11b+ cells after isolation. Right plot: Cells before isolation. Cells were stained with anti-mouse CD11b+ (clone M1/70) APC, Ly-6G/Ly-6C (Gr-1) PE. Dead cells were excluded by Helix NP™ Blue.
  • MojoSort_Mouse_CD11b_Selection_Kit_2_082820
    A single cell suspension from C57BL/6 mouse brain was prepared for the positive selection of CD11b+ cells using the MojoSort™ Mouse CD11b+ Selection Kit. Right plot: CD11b+ cells after isolation. Right plot: Cells before isolation. Cells were stained with anti-mouse CD11b+ (clone M1/70) APC and P2RY12 (clone S16007D) PE. Dead cells were excluded by Helix NP™ Blue.
Cat # Size Price Quantity Avail. Save
480109 20 tests 168€
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480110 200 tests 516€
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Description

Mouse CD11b+ cells are either selected or depleted by incubating the sample with a biotin conjugated anti-mouse CD11b+ antibody and then magnetic streptavidin nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD11b+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat. No. 480109:

  • 1 vial containing 200 µL of biotin anti-mouse CD11b+ antibody (clone M1/70)
  • 1 vial containing 200 µL of streptavidin nanobeads


For Cat. No. 480118:

  • 2 vials containing 1 mL each of biotin anti-mouse CD11b+ antibody (clone M1/70)
  • 2 vials containing 1 mL each of streptavidin nanobeads

Product Details

Reactivity
Mouse
Formulation
Antibody: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2 with stabilizer.
Streptavidin nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
The antibodies were purified by affinity chromatography, and conjugated wtih biotin under optimal conditions.
Streptavidin nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

Volume of biotin antibody and streptavidin nanobeads should be adjusted depending on the different tissue of CD11b+ cells to be isolated. Use the table below as an example when working with different mouse tissue. For 1x107 cells in 100 µL of buffer, use the following volumes:
 

Tissue

Biotin Antibody

Optimal Nanobeads Volume

Mouse Bone Marrow

10 µL

20 µL

Mouse Splenocytes

5 µL

10 µL

Mouse Lung

10 µL

10 µL

Mouse Brain

10 µL

2 µL


The volumes indicated in the table are for the use of MojoSort™ magnet (Cat. Nos. 480109 & 480110). For low frequency cells, pre-dilute the streptavidin nanobeads in order to pipette a minimum of 5 µL of any solution. Avoid working with small volumes.

Additional Product Notes

This kit is designed for the positive selection or depletion of mouse CD11b+ cells from mouse bone marrow, lymphoid tissue, lung and brain, among other organs.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.

To find out the dilution to use for this product in separation columns please download the CoA.

This kit is not recommended for CD11b positive selection from human samples based on in-house testing results.

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Go To Top Version: 3    Revision Date: 05.25.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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