MojoSort™ Mouse CD117 (c-Kit) Selection Kit

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MojoSort_Mouse_CD117_Selection_Kit_1_010719
A single cell suspension from C57BL/6 mouse bone marrow was prepared for the positive selection of CD117 (c-Kit)+ cells using the MojoSort™ Mouse CD117 (c-Kit) Selection Kit. The cells were incubated with TruStain FcX™ (anti-mouse CD16/32) antibody prior to the addition of the anti-mouse CD117 (c-Kit) PE antibody. After separation, the cells were stained with APC anti-mouse CD117 (c-Kit) (clone ACK2) and Brilliant Violet 421™ anti-mouse lineage cocktail (clones 17A2/RB6-8C5/RA3-6B2/Ter-119/M1/70). Dead cells were excluded by 7-AAD.
  • MojoSort_Mouse_CD117_Selection_Kit_1_010719
    A single cell suspension from C57BL/6 mouse bone marrow was prepared for the positive selection of CD117 (c-Kit)+ cells using the MojoSort™ Mouse CD117 (c-Kit) Selection Kit. The cells were incubated with TruStain FcX™ (anti-mouse CD16/32) antibody prior to the addition of the anti-mouse CD117 (c-Kit) PE antibody. After separation, the cells were stained with APC anti-mouse CD117 (c-Kit) (clone ACK2) and Brilliant Violet 421™ anti-mouse lineage cocktail (clones 17A2/RB6-8C5/RA3-6B2/Ter-119/M1/70). Dead cells were excluded by 7-AAD.
  • MojoSort_Mouse_CD117_Selection_Kit_2_010719
    A single cell suspension from C57BL/6 mouse bone marrow was prepared for the positive selection of CD117 (c-Kit)+ cells. The MojoSort™ Mouse Hematopoietic Progenitor Cell Isolation Kit (Cat. No. 480003/480004) was first used to remove Lin+ cells. The MojoSort™ Mouse CD117 (c-Kit) Selection Kit was then used to further enrich for CD117 (c-kit)+ cells. The cells were incubated with TruStain FcX™ (anti-mouse CD16/32) antibody prior to the addition of the PE anti-mouse CD117 (c-Kit) antibody. After separation, the cells were stained with APC anti-mouse CD117 (c-Kit) (clone ACK2) and Brilliant Violet 421™ anti-mouse lineage cocktail (clones 17A2/RB6-8C5/RA3-6B2/Ter-119/M1/70). Dead cells were excluded by 7-AAD.
Cat # Size Price Quantity Avail. Save
480145 20 tests 124€
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480146 200 tests 436€
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Description

Mouse CD117 (c-Kit)+ cells are either selected or depleted by incubating the sample with a PE conjugated anti-mouse CD117 (c-Kit) antibody and then magnetic Mouse anti-PE Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD117 (c-Kit)+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480145:

•  1 vial containing 100 μL of TruStain FcX™ (anti-mouse CD16/32) antibody

•  1 vial containing 200 µL of PE anti-mouse CD117 (c-Kit) antibody (clone 2B8)

•  1 vial containing 200 µL of Mouse anti-PE Nanobeads

 

For Cat# 480146:

•  1 vial containing 1 mL of TruStain FcX™ (anti-mouse CD16/32) antibody

•  2 vials containing 1 mL each of PE anti-mouse CD117 (c-Kit) antibody (clone 2B8)

•  2 vials containing 1 mL each of Mouse anti-PE Nanobeads

Product Details

Reactivity
Mouse
Formulation
Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2 with stabilizer.
Particle: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Conjugated nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µL of PE anti-mouse CD117 (c-Kit) antibody for 1 x 107 cells in 100 µL of buffer.

10 µL of Mouse anti-PE Nanobeads for 1 x 107 cells in 100 µL of buffer.

Application Notes

This kit is designed for the positive selection or depletion of mouse CD117 (c-Kit)+ cells from mouse bone marrow.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.


Antibody or cocktail dilution to use in column: 4x
Nanobead dilution to use in column: 8x

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Go To Top Version: 2    Revision Date: 05.25.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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