- Product Information
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.
The polyclonal antibody Poly19051 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Loricrin. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
- Human, Mouse
- This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse loricrin protein. It was subsequently purified using peptide affinity chromatography.
- Phosphate-buffered solution+ 0.03% Thimerosal.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- When stored at ≤ -20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• IHC: 1:1,000 with either biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).
Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (Cat. No. 928502, 928602).
Incubation: 60 minutes at room temperature
Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
(PubMed link indicates BioLegend citation)
- Wallace L, Roberts-Thompson L, and Reichelt J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci., 125: 1750-1758, Apr 2012. PubMed
- Bayo P, Sanchis A, Bravo A, Cascallana JL, Buder K, Tuckermann J, Schütz G, and PTrez P. Glucocorticoid Receptor Is Required for Skin Barrier Competence. Endocrinology, 149: 1377-1388, Mar 2008. [IHC] PubMed
- Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001.
- Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.
- Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984. 6. Suzuki K, et al. 2009. Development. 136:367. (IHC) PubMed
- Croyle MJ, et al. Role of epidermal primary cilia in the homeostasis of skin and hair follicles. Development, 138: 1675-1685, May 2011. [IHC, IF] PubMed
- Sengupta A, et al. 2010. PLoS One. 5:12249. (IHC) PubMed
- Suzuki K, et al. 2009. Development. 136:367. (IHC) PubMed
- Liakath-Ali K, et al. 2014. Nat Commun. 11:3540. (IHC) PubMed
- Product Citations
AB_2565046 (BioLegend Cat. No. 905101)
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