- Other Names
- Multiplex assay, Flow cytometry, LEGENDplex
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|740579||100 tests (3.5 mL)||200€|
The LEGENDplex™ Human Kidney Function Panel 1, for Urine Samples Detection Antibodies will be used together with the following Mix and Match Capture Beads, Human Kidney Function Panel 1, and Urine Samples Standard to offer flexible customization within the panel:
740580 (LEGENDplex™ Human Kidney Function Panel 1, for Urine Samples Standard)
740539 (LEGENDplex™ Buffer Set G)
740472 (LEGENDplex™ Human IGFBP7 Capture Bead A4, 13X)
740567 (LEGENDplex™ Human β2-microglobulin Capture Bead A5, 13X)
740568 (LEGENDplex™ Human Cystatin C Capture Bead A6, 13X)
740569 (LEGENDplex™ Human Apo J (Clusterin) Capture Bead A7, 13X)
740570 (LEGENDplex™ Human EGF Capture Bead A8, 13X)
740571 (LEGENDplex™ Human Albumin Capture Bead B3, 13X)
740572 (LEGENDplex™ Human Osteopontin Capture Bead B4, 13X)
740573 (LEGENDplex™ Human TFF3 Capture Bead B7, 13X)
740574 (LEGENDplex™ Human Uromodulin Capture Bead B9, 13X)
740377 or 740379 (Filter Plate or V-bottom Plate for LEGENDplex™ Assay)
The LEGENDplex™ Human Kidney Function Panel 1 (9-plex), for Urine Samples is a multiplex bead-based assay, which uses fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 9 human proteins, including IGFBP7, B2M, Cystatin C, Clusterin, EGF, Albumin, OPN, TFF3, and Uromodulin. This assay panel provides high detection sensitivities and broad dynamic ranges.
- Molecular Family
- Enzymes and Regulators, Growth Factors, Cytokines/Chemokines
- Gene ID
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.