- Regulatory Status
- Other Names
- IL-1 beta, IL-1β, IFN-alpha2, IFN-α2, IFN-gamma, IFN-γ, TNF-α, TNF-alpha, MCP-1, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-18, IL-23, IL-33
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- Product Citations
Inflammation is a biological response triggered by exposure to harmful stimuli such as tissue damage or foreign antigens. This response is initiated by resident immune cells, and then mediated through the actions of various cytokines and chemokines. Abnormalities in inflammation also underlie a large variety of chronic human diseases. Accurate measurement of inflammatory mediators is therefore critical to further our understanding of the role these molecules play in shaping the immune response to injury or infection.
The Human Inflammation Panel 1 is a bead-based multiplex assay, using fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 human inflammatory cytokines/chemokines, including IL-1β, IFN-α2, IFN-γ, TNF-α, MCP-1 (CCL2), IL-6, IL-8 (CXCL8), IL-10, IL-12p70, IL-17A, IL-18, IL-23, and IL-33. This assay panel provides higher detection sensitivitiy and broader dynamic range than traditional ELISA methods. The panel has been validated for use on serum and cell culture supernatant samples.
The Human Inflammation Panel 1 is designed to allow flexible customization within the panel. Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel.
This assay is for research use only.
- Kit Contents
- Setup Beads 1: FITC Beads (1 ml)
- Setup Beads 2: PE Beads (1 ml)
- Setup Beads 3: Raw Beads (2 ml)
- Capture Beads
- Detection Antibody (3.5 ml)
- Standard Cocktail, Lyophilized (1 vial)
- SA-PE (3.5 ml)
- Matrix B3, Lyophilized (1 vial)
- Assay Buffer (25 ml)
- Wash Buffer, 20X (25 ml)
- Filter Plate (1 plate)
- Plate Sealers (4 sheets)
Please note: Lots manufactured before September 2021 may include a different version of the “Matrix B.” There is no change in the protocol. Simply use the Matrix reagent you received as suggested in the current manual.
- Verified Reactivity
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here.
- Product Citations
- Materials Not Included
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes, in 96-Tube Rack
- Vortex Mixer
- Aluminum foil
- Absorbent pads
- Plate Shaker
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- Biology Area
- Cell Biology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Gene ID
- 3553 View all products for this Gene ID 3439 View all products for this Gene ID 3440 View all products for this Gene ID 3458 View all products for this Gene ID 7124 View all products for this Gene ID 3569 View all products for this Gene ID 3576 View all products for this Gene ID 3586 View all products for this Gene ID 3592 View all products for this Gene ID 3593 View all products for this Gene ID 3605 View all products for this Gene ID 3606 View all products for this Gene ID 51561 View all products for this Gene ID 90865 View all products for this Gene ID 6347 View all products for this Gene ID
- View information about IL-1beta, IFN-alpha2, IFN-gamma, TNF-alpha, MCP-1 CCL2, IL-6, IL-8 CXCL8, IL-10, IL-12p70, IL-17A, IL-18, IL-23, IL-33 on UniProt.org
- If I don't have a vacuum, how do I remove the liquid from my plate?
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.