- Other Names
- Multiplex assay, Flow cytometry, LEGENDplex
- Ave. Rating
- Submit a Review
- Product Citations
The LEGENDplex™ Human B Cell Panel Standard can be used together with the following Mix and Match Capture Beads and Human B Cell Panel Detection Antibodies to offer flexible customization within the panel (13-plex):
740536 (LEGENDplex™ Human B Cell Panel Detection Antibodies)
740538 (LEGENDplex™ Human TNF-α Capture Beads A4, 13X)
740047 (LEGENDplex™ Human IL-13 Capture Beads A5, 13X)
740540 (LEGENDplex™ Human IL-4 Capture Beads A6, 13X)
740541 (LEGENDplex™ Human IL-10 Capture Beads A7, 13X)
740542 (LEGENDplex™ Human IL-6 Capture Beads A8, 13X)
740543 (LEGENDplex™ Human IL-2 Capture Beads A10, 13X)
740544 (LEGENDplex™ Human TNF-β Capture Beads B2, 13X)
740545 (LEGENDplex™ Human IFN-γ Capture Beads B3, 13X)
740546 (LEGENDplex™ Human IL-17A Capture Beads B4, 13X)
740547 (LEGENDplex™ Human IL-12p70 Capture Beads B5, 13X)
740548 (LEGENDplex™ Human APRIL Capture Beads B6, 13X)
740549 (LEGENDplex™ Human BAFF Capture Beads B7, 13X)
740550 (LEGENDplex™ Human CD40L Capture Beads B9, 13X)
740377 or 740379 (Filter Plate or V-bottom Plate for LEGENDplex™ Assay)
740525 (LEGENDplex™ Buffer Set A1)
- Biology Area
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Gene ID
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.