ELISA MAX™ Deluxe Set Human IL-2

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-2 ELISA Kit
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Product Citations
publications
human il-2_122109
  • human il-2_122109
Cat # Size Price Quantity Check Availability Save
431804 5 plates 229€
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431815 10 Plates 440€
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431816 20 Plates 704€
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Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Human IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human IL-2 ELISA MAX™ Capture Antibody (200X)
  • Human IL-2 ELISA MAX™ Detection Antibody (200X)
  • Human IL-2 Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

The assay kits will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

Application References

(PubMed link indicates BioLegend citation)
  1. Poirier N, et al. 2010. Sci Transl Med. 2:17ra10. (Deluxe kit) PubMed
  2. Redkiewicz P, et al. 2012. Plant Biotechnol J. 10:806. PubMed
  3. Sturner KH, et al. 2014. J Immunol. 193:4439. PubMed
  4. Albring A, et al. 2014. Pharmacol Biochem Behav. 16:126. PubMed
Product Citations
  1. Redkiewicz P, et al. 2012. Plant Biotechnol J. 10:806. PubMed
  2. Stürner K, et al. 2014. J Immunol. 193:4439. PubMed
  3. Albring A, et al. 2014. Pharmacol Biochem Behav. 16:126. PubMed
  4. Karampetsou M, et al. 2016. J Immunol. 196: 4915 - 4924. PubMed
  5. Zhang Y, et al. 2020. Int J Mol Sci. 21:00. PubMed
  6. Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed
  7. Dammermann W, et al. 2017. J Virol Methods. 10.1016/j.jviromet.2017.07.011. PubMed
  8. Tello‐Lafoz M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16370-w. PubMed
  9. Bröker K, et al. 2019. J Transl Med. 17:6. PubMed
  10. Trinklein ND, et al. 2019. MAbs. 11:639. PubMed
  11. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
Sensitivity
4 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2 SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2 HPO4 , 0.2 g KH2 PO4 , 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Sources
T cells
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
3558 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 6    Revision Date: 07.20.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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