ELISA MAX™ Deluxe Set Human IFN-γ

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human ifn-g_121609
  • human ifn-g_121609
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430104 5 plates 244€
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430115 10 Plates 469€
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430116 20 Plates 751€
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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human IFN-γ ELISA MAX™ Capture Antibody (200X)
  • Human IFN-γ ELISA MAX™ Detection Antibody (200X)
  • Human IFN-γ Standard
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Older lots of these products may contain Substrate Solutions A and B instead of Substrate Solution D mentioned on the TDS and the current manual. Please refer to the lot-specific CoA for the list of the reagents included in the lot and follow the ELISA procedure provided there.

Product Details

Verified Reactivity


As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

The assay kits will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

Application References

(PubMed link indicates BioLegend citation)
  1. Ye Z, et al. 2008. Infect Immun. 75:2541. PubMed
  2. Zhang Y, et al. 2010. Infect Immun. 78:2329. PubMed
  3. Peng J, et al. 2005. J. Immunother. 28:599. PubMed
  4. Bendersky A, et al. 2012. J. Immunol. 188:4349. PubMed
  5. Boggio E, et al. 2012. Hum Immunol. 73:585. PubMed
  6. Ankrum JA, et al. 2014. Sci Rep. 4:4645. PubMed
  7. Wu Z, et al. 2015. J Virol. 89:2906. PubMed
Product Citations
  1. Wu Z, et al. 2015. J Virol. 89:2906. PubMed
  2. Klement JD, et al. 2018. J Clin Invest. 128:5549. PubMed
  3. Cordoba S, et al. 2021. Nat Med. 27:1797. PubMed
  4. Scurr MJ, et al. 2022. Nat Commun. 13:5422. PubMed
  5. Bröker K, et al. 2019. J Transl Med. 17:6. PubMed
  6. Kilcollins A, et al. 2016. J Immunol. 197: 419 - 428. PubMed
  7. Meryk A et al. 2019. Cell reports. 26(10):2681-2691 . PubMed
  8. Pires D, et al. 2021. Front Immunol. 12:742822. PubMed
  9. Singh N, et al. 2018. PLoS One. 13:e0199817. PubMed
  10. Singhal J, et al. 2022. Front Cell Infect Microbiol. 12:878136. PubMed
  11. Wang H, et al. 2021. J Immunother Cancer. 9:. PubMed
  12. Boggio E, et al. 2012. Hum Immunol. 73:585. PubMed
  13. Bendersky A, et al. 2012. J Immunol. 188:4349. PubMed
  14. Ye Z, et al. 2008. Infect Immun. 76:2541. PubMed
  15. Ollé Hurtado M, et al. 2019. PLoS One. 14:e0216373. PubMed
  16. Booty MG, et al. 2022. J Immunol. 208:929. PubMed
  17. Ankrum J, et al. 2014. Sci Rep. 4:4645. PubMed
  18. You Y, et al. 2021. Mol Med Rep. 24:. PubMed
  19. Karampetsou M, et al. 2016. J Immunol. 196: 4915 - 4924. PubMed
  20. Aldeghaither DS, et al. 2019. Cancer Immunol Res. 7:230. PubMed
  21. Kofla G, et al. 2022. Oncoimmunology. 11:2068109. PubMed
  22. Godbersen C, et al. 2017. Mol Cancer Ther. 16:1335. PubMed
  23. Sp N, et al. 2021. Int J Mol Sci. 22:. PubMed
  24. Hirschberger S, et al. 2022. Front Med (Lausanne). 9:923502. PubMed
  25. Liu CA, et al. 2022. Cancers (Basel). 14:. PubMed
  26. Madison BB, et al. 2022. Mol Ther Nucleic Acids. 29:979. PubMed
  27. Fernandes RA, et al. 2020. Nature. 779:586. PubMed
  28. Bliska Y 2010. Infect Immun. 78:2359. PubMed
  29. Leung EYL, et al. 2020. Mol Ther Oncolytics. 16:289. PubMed
  30. Wu Z, et al. 2019. Microorganisms. 0.670833333. PubMed
  31. Trinklein ND, et al. 2019. MAbs. 11:639. PubMed
  32. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
  33. Xie X, et al. 2021. Front Immunol. 12:625808. PubMed
  34. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  35. Subramanian N, et al. 2017. J Gen Virol. 98(12). PubMed
  36. Pires D, et al. 2021. Front Immunol. 12:647728. PubMed
  37. Kang CH, et al. 2020. Int J Mol Sci. 21:00. PubMed
  38. Zhang X, et al. 2019. Hematol Oncol. 37(3):270. PubMed
  39. Iraqi M, et al. 2022. Int J Mol Sci. 23:. PubMed
  40. Pierini S, et al. 2020. JCI Insight. 5:00. PubMed
  41. Dammermann W, et al. 2017. J Virol Methods. 10.1016/j.jviromet.2017.07.011. PubMed
  42. Singh SS, et al. 2022. Clin Transl Immunology. 11:e1396. PubMed
  43. Wang E, et al. 2015. Cancer Immunol Res. 3: 815 - 826. PubMed
  44. Kunyanee C, et al. 2016. PLoS One. 11: 0160741. PubMed
  45. Lei W, et al. 2022. Blood Cancer J. 12:35. PubMed
  46. Lucchesi D, et al. 2020. Arthritis Rheumatol. 1559:72. PubMed
  47. Singh N, et al. 2017. Mol Immunol. 85:111. PubMed
4 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Type
Biology Area
Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Gene ID
3458 View all products for this Gene ID
View information about IFN-gamma on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 6    Revision Date: 07.15.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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