Human β-Amyloid Peptide (1-42)

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Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
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Human_beta-Amyloid_peptide_1-42_041618
Direct ELISA of HRP anti-β-Amyloid, 1-16 antibody (clone 6E10; Cat. No. 803011), HRP anti-β-Amyloid, 1-42 antibody (clone 12F4; Cat. No. 805507), and HRP anti-β-Amyloid, 1-40 antibody (clone 11A50-B10; Cat. No.805407) binding to plate-immobilized Human β-Amyloid Peptide (1-42). ELISA was performed by coating wells with 10 ng of Human β-Amyloid Peptide (1-42). The wells were then incubated with the primary antibodies at 37°C for 45 minutes. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
  • Human_beta-Amyloid_peptide_1-42_041618
    Direct ELISA of HRP anti-β-Amyloid, 1-16 antibody (clone 6E10; Cat. No. 803011), HRP anti-β-Amyloid, 1-42 antibody (clone 12F4; Cat. No. 805507), and HRP anti-β-Amyloid, 1-40 antibody (clone 11A50-B10; Cat. No.805407) binding to plate-immobilized Human β-Amyloid Peptide (1-42). ELISA was performed by coating wells with 10 ng of Human β-Amyloid Peptide (1-42). The wells were then incubated with the primary antibodies at 37°C for 45 minutes. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
Cat # Size Price Quantity Avail. Save
932501 500 µg 152€
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932502 1 mg 300€
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Description

Amyloid Beta (Aβ) peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons. Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Chemically Synthesized
Molecular Mass
4514.1
Purity
≥95%, % Peak Area by HPLC
Formulation
Lyophilized white powder
Storage & Handling
Peptide is shipped at ambient temperature. Upon receipt, store lyophilized powder at ≤ -20°C. Reconstituted peptide should be aliquoted and stored at ≤ -20°C. Do not freeze/thaw.
Peptide Reconstitution: It is recommended to use 1% NH4OH as the solvent followed by immediate dilution in buffer (i.e. PBS). Add 1% NH4OH directly to the lyophilized powder (add 35-40 µL to 0.5 mg or 70-80 µL to 1.0 mg), then immediately dilute the solution in your buffer to a concentration of 1.0 mg/mL or less. The peptide cannot be stored in 1% NH4OH long-term.
Application

Direct ELISA Assay

Recommended Usage

Validated in Direct ELISA as coating peptide.

The peptide may also be useful in additional applications such as Western Blot and Immunoprecipitation. Optimal conditions to be determined by end user.

Application Notes

Direct ELISA: Peptide Coat Concentration 0.2 mg/mL

Antigen Details

Structure
Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue sources: Primarily nervous system, but also adipose tissue, intestine, muscle.
Distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1
  3. Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83.
  4. Puig KL, et al. 2012.  Exp. Gerontol. 48(7): 608.
  5. Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595.
  6. Walsh DM, et al.  2007. J. Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid (1-42) on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 04.16.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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