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Human Adiponectin Protein (Acrp30) (ELISA Std.)

Pricing & Availability
Regulatory Status
RUO
Other Names
GBP-28, apM1, AdipoQ, and Acrp30
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Product Citations
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558409 4 pack 76€
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Description

Adiponectin, also referred as Acrp30, AdipoQ, apM1, and gelatin binding protein of 28 kD (GBP-28), is encoded by the ADIPOQ gene in humans. Adiponectin contains 244 amino acids with a collagen-like domain at the N-terminal and a globular domain at the C-terminal. The globular domain can be proteolytically truncated forming the globular adiponectin (gAdiponectin). This globular domain resembles the structure of the complement C1q and the TNF-α superfamily. Adiponectin exists in a wide range of homo-oligomers: a low-molecular weight (LMW) trimer, a medium molecular weight (MMW) hexamer, and a high molecular weight (HMW) multimer with little or no interconversion between the forms. Females generally have higher HMW than males in proportion and absolute amounts. Adiponectin has two receptors (adipoRs)-1 and -2. AdipoR1 mRNA is detectable in skeletal muscle, spleen, lung, heart, kidney, and liver. AdipoR2 is expressed in liver, but is also detectable in heart, lung, skeletal muscle, and kidney.

Adiponectin plays an important role in carbohydrate and lipid metabolism. It promotes insulin-sensitivity and protects cells from inflammation and apoptosis. It is also hypothesized to have anti-atherogenic activity. Normal circulating adiponectin level ranges from 4-30 µg/mL. Adiponectin levels are inversely correlated with body mass index (BMI). Low serum level of adiponectin is found in obesity, type 2 diabetes and those with coronary artery disease. Therefore, adiponectin serves as an important clinical biomarker for metabolic syndromes.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human pooled serum.
Molecular Mass
The 226 amino acid protein has a predicted molecular mass of approximately 24.5 kD (calculated without glycosylation).
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Storage & Handling
Immediately upon receipt, store unopened vials between 2°C and 8°C and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA - Quality tested
Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 10,000 to 156 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This Adiponectin protein is useful as a standard for a human Adiponectin (Acrp30) sandwich ELISA, using purified clone 62-9 antibody for capture and biotinylated clone 112-1 antibody for detection.

Antigen Details

Structure
Protein hormone.
Distribution

Adipose tissue.

Function
Modulates a number of metabolic processes including glucose regulation and fatty acid oxidation.
Interaction
Liver, heart, lung, skeletal muscle, and kidney.
Ligand/Receptor
Adiponectin receptor 2
Biology Area
Complement
Antigen References

1. Ouchi N, et al. 2000. Circulation 102:1296.
2. Ouchi N, et al. 1999. Circulation 100:2473-6.
3. Nakano Y, et al. 1996. J. Biochem. 120:803.
4. Yokota T, et al. 2000. Blood 96:1723.

Gene ID
9370 View all products for this Gene ID
UniProt
View information about Adiponectin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02.29.2016

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