HRP Goat anti-mouse IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4053 (See other available formats)
Isotype
Goat Polyclonal IgG
Ave. Rating
2 reviews
Product Citations
publications
Cat # Size Price Quantity Avail. Save
405306 500 µl 96€
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Description

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Aqueous buffered solution containing a carrier protein and preservatives.
Preparation
The polyclonal antibody was purified from goat antiserum by mouse immunoglobulin affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
This product is guaranteed for six months. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product
Application

ELISA - Quality tested
WB, IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. Do not use this product beyond the expiration date. For ELISA or Western blot analysis, the reagent should be titrated between 1:2,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase.

Application Notes

This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Scotta, C, et al. 2008. J. Immunol. 181:1025. PubMed
Product Citations
  1. Frank N, et al. 2011. Cancer Res. 71:1474. PubMed
  2. Hwang S, et al. 2012. Proc Natl Acad Sci U S A. 110:276. PubMed
  3. Horton N, et al. 2013. PLoS One. 8:e59552. PubMed
  4. Hsing C, et al. 2008. Cytokine. 44:221. PubMed
  5. Yan L, et al. 2014. Clin Vaccine Immunol. 21:1206. PubMed
  6. Copp J, et al. 2014. Proc Natl Acad Sci U S A. 111:13481. PubMed
  7. Collin R, et al. 2014. J Immunol. 193:3503. PubMed
  8. Shkoporov A, et al. 2015. FEMS Microbiol Lett. 362:83. PubMed
  9. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  10. Sertorio M, et al. 2016. J Immunol. 196: 2986-2994. PubMed
  11. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  12. Vrieling M, et al. 2016. Sci Rep. 6:37759. PubMed
  13. Ortega-Prieto AM, et al. 2018. Nat Commun. 9:682. PubMed
  14. Miyamoto M, et al. 2018. Oncol Lett. 15:4005. PubMed
  15. Chen G, et al. 2018. Front Immunol. 9:549. PubMed
  16. Omer S 2018. eLife. 7: e36745. PubMed
  17. Wang C, et al. 2019. PLoS Pathog. 15:e1007938. PubMed
  18. Chung KP, et al. 2019. Nat Commun. 10:3390. PubMed
Publication Library
RRID
AB_315009 (BioLegend Cat. No. 405306)

Related FAQs

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