Western blot of HRP anti-Myelin Protein Zero (clone 58A). Lane 1: Molecular weight marker; Lane 2: 5 µg of mouse sciatic nerve lysate; Lane 3: 10 µg of rat sciatic nerve lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
Western blot of HRP anti-Myelin Protein Zero (clone 58A). Lane 1: Molecular weight marker; Lane 2: 5 µg of mouse sciatic nerve lysate; Lane 3: 10 µg of rat sciatic nerve lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
IHC staining of HRP anti-Myelin Protein Zero (clone 58A) on formalin-fixed paraffin-embedded rat sciatic nerve tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of HRP anti-Myelin Protein Zero (clone 58A) on formalin-fixed paraffin-embedded mouse sciatic nerve tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of HRP anti-Myelin Protein Zero (clone 58A) on formalin-fixed paraffin-embedded human dorsal root ganglia tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Myelin protein zero (MPZ), is a membrane glycoprotein mainly expressed in the peripheral nervous system (PNS). It is the major structural component of the myelin sheath, and accounts for at least 50% of all proteins in the PNS. MPZ is expressed by Schewann cells. Mutations in MPZ have been associated with myelin deficiency, which may lead to neuropathies including charcot-Marie-Tooth and Dejerine-Sottas diseases.
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
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