Go-ChIP-Grade™ Purified anti-p53 Antibody

Pricing & Availability
Clone
DO-1 (See other available formats)
Other Names
Tumor protein p53 (TP53)
Isotype
Mouse IgG2a, κ
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Product Citations
publications
1-DO-1_ChIP_p53_Antibody_030320_updated.png
Chromatin Immunoprecipitations (ChIP) were done with fixed and sonicated chromatin samples from 293T cells treated with tunicamycin (2 µg/mL, 8 hr). ChIP was performed with 5 µg of chromatin and either 1 µg of Go-ChIP-Grade™ purified anti-p53 antibody (Clone DO-1), or equal amount of Go-ChIP-Grade™ purified mouse IgG2a, κ isotype ctrl antibody (Clone MG2a-53, Cat. No. 401506). The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for the human CDKN1A promoter, and for a gene desert region on human chromosome 12. The amount of immunoprecipitated DNA in each sample is represented as a percentage of the total amount of input chromatin, which is equivalent to 100%.
  • 1-DO-1_ChIP_p53_Antibody_030320_updated.png
    Chromatin Immunoprecipitations (ChIP) were done with fixed and sonicated chromatin samples from 293T cells treated with tunicamycin (2 µg/mL, 8 hr). ChIP was performed with 5 µg of chromatin and either 1 µg of Go-ChIP-Grade™ purified anti-p53 antibody (Clone DO-1), or equal amount of Go-ChIP-Grade™ purified mouse IgG2a, κ isotype ctrl antibody (Clone MG2a-53, Cat. No. 401506). The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for the human CDKN1A promoter, and for a gene desert region on human chromosome 12. The amount of immunoprecipitated DNA in each sample is represented as a percentage of the total amount of input chromatin, which is equivalent to 100%.
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645703 25 µg 76€
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645704 100 µg 180€
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Description

p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
P53 N-terminal linear epitope aa 20-25
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested

Recommended Usage

The recommended starting antibody to chromatin ratio is 1 µg of antibody and 5 µg of chromatin per IP. Optimal antibody to chromatin ratio should be determined by the end user. It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application References

(PubMed link indicates BioLegend citation)
  1. Vojtesek B, et al. 1992. J Immuno Methods 151:237
  2. Stephen CW, et al. 1995. J Mol Biol 248:58
RRID
AB_2832839 (BioLegend Cat. No. 645703)
AB_2832840 (BioLegend Cat. No. 645704)

Antigen Details

Structure
Tetramer; 53 kD
Distribution

Nuclear when activated

Function
Tumor suppressor, transcription factor regulates cell cycle checkpoint, apoptosis, DNA integrity
Interaction
mdm2, mdmx, TopoI, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, HDAC1-3; mutant p53 associates with p63, p73
Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, DNA Repair/Replication, Stem Cells, Transcription Factors
Antigen References

1. Vogelstein B, et al. 1992. Cell. 70:523.
2. Shieh S, et al. 1997. Cell. 91:325.
3. Mihara M, et al. 2003. Mol. Cell 11:577.
4. Saito S, et al. 2003. J. Biol. Chem. 278:37536.

Gene ID
7157 View all products for this Gene ID
UniProt
View information about p53 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04.14.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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