Go-ChIP-Grade™ Purified anti-IRF9 Antibody

Pricing & Availability
Clone
5A3A39 (See other available formats)
Other Names
Interferon regulatory factor 9 (IRF-9), Interferon-stimulated gene factor 3 gamma (ISGF-3 gamma), ISGF3 p48 subunit
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1_5A3A39_GoChIP_IRF9_Antibody_110617
Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from THP-1 cells treated with IFNα2 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-IRF9 (Clone 5A3A39), or B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MX1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 1_5A3A39_GoChIP_IRF9_Antibody_110617
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from THP-1 cells treated with IFNα2 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-IRF9 (Clone 5A3A39), or B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MX1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 2_5A3A39_Purified_IRF9_Antibody_Updated_103017
    Total lysates (15 µg protein) from Jurkat (lane 1), Jurkat treated with IFNα1 (lane 2), THP-1 (lane 3) and Raw264.7 cells (lane 4) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1250 diluted (0.4 µg/mL) Purified anti-IRF9 Antibody, clone 5A3A39 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-IRF9 Antibody or a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH Antibody. (lower). Lane M: Molecular weight ladder.
  • 3_5A3A39_Purified_IRF9_Antibody_IF_060614
    IFN-α treated Jurkat cells were stained with purified anti-IRF9 (5A3A39) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
Cat # Size Price Quantity Avail. Save
660703 25 µg 60€
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660704 100 µg 188€
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Description

IRF9 is a member of the IRF family of transcription factors and plays an important role in IFN signaling pathways and anti-viral immune responses. Type I and type III interferon stimulation induces phosphorylation and heterodimerization of STAT1 and STAT2. The activated STAT1:STAT2 heterodimer recruits IRF9 and forms a heterotrimeric complex termed IFN-stimulated gene factor 3 (ISGF3). The ISGF3 complex, in turn, translocates to the nucleus and transactivates IFN-inducible genes. The IRF9 protein in the ISGF3 complex is responsible for the recognition of IFN-stimulated response elements (ISRE). Mice lacking IRF9 fail to induce the expression of IFN regulated genes in response to type I interferon stimulation, indicating that IRF9 is crucial in mediating interferon signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human IRF9 recombinant protein (116-387 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB, ICC - Verified 

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1-50 to 1-200 by volume. For Western blotting, the suggested use of this reagent is 0.4 - 2.5 µg per mL. It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

This clone does not react with mouse (in-house tested). A non-specific band was observed (around 37 KD) when this antibody was tested on Raw264.7 cells

RRID
AB_2721591 (BioLegend Cat. No. 660703)
AB_2721592 (BioLegend Cat. No. 660704)

Antigen Details

Structure
393 amino acids, predicted molecular weight of 44 kD; contains a IRF tryptophan pentad repeat responsible for DNA binding.
Distribution

Cytoplasm, phosphorylated form translocated to the nucleus.

Function
IRF9 is a transcription factor that mediates interferon signaling and activates the expression of interferon inducible genes.
Interaction
Forms the interferon-stimulated gene factor 3 complex (ISGF3) with Signal Transducer and Activator of Transcription 1 (STAT1) and STAT2 in response to type I IFN stimulation.
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Fink K and Grandvaux N, 2013. JAKSTAT. 2:e27521. 
2. Tsuno T, et al. 2009. J. Immunother. 32:803. 
3. Thibault DL, et al. 2008. J. Clin. Invest. 118:1417. 
4. Kraus TA, et al. 2003. J. Biol. Chem. 278:13033. 
5. Xiao W, et al. 2001. J. Biol. Chem. 276:23275. 
6. Veals SA, et al. 1992. Mol. Cell. Biol. 12:3315.

Gene ID
10379 View all products for this Gene ID
UniProt
View information about IRF9 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03.15.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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