Go-ChIP-Grade™ Purified anti-HDAC2 Antibody

Pricing & Availability
Clone
3F3/HDAC2 (See other available formats)
Other Names
Histone deacetylase 2, YY1-associated factor 1, transcriptional regulator homolog RPD3, YAF1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_3F3slashHDAC2_GoChIP_HDAC2_Antibody_012717
Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from Jurkat cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-HDAC2 (clone 3F3/HDAC2), B) equal amount of Purified Mouse IgG1 Isotype Control Antibody, or C) competitor's ChIP-grade Purified anti-HDAC2 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human FOSL1 (Fos like 1) gene region, which is known to be bound to HDAC2. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 1_3F3slashHDAC2_GoChIP_HDAC2_Antibody_012717
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from Jurkat cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-HDAC2 (clone 3F3/HDAC2), B) equal amount of Purified Mouse IgG1 Isotype Control Antibody, or C) competitor's ChIP-grade Purified anti-HDAC2 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human FOSL1 (Fos like 1) gene region, which is known to be bound to HDAC2. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 2_3F3slashHDAC2_Purified_HDAC2_Antibody_1_082918
    IHC staining of purified anti-HDAC2 antibody (clone 3F3/HDAC2) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_3F3slashHDAC2_Purified_HDAC2_Antibody_2_082918
    Western blot of purified anti-HDAC2 antibody (clone 3F3/HDAC2). Lane 1: Molecular weight marker; Lane 2: 20 µg of HeLa cell lysate; Lane 3: 20 µg of 3T3 cell lysate; Lane 4: 20 µg of PC12 cell lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
815203 25 µg 60€
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815204 100 µg 188€
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. The purified format (Cat. No. 815201) can be used as an alternate.

Description

HDAC2 is a member of class I histone deacetylase (HDACs) family, which modulates the chromatin structure by removing acetyl groups (deacetylation) from the side chain of lysine residues on the N-terminal region of core histones (H2A, H2B, H3 and H4). Generally, histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.

HDAC2 lacks DNA binding activity and executes its function by recruiting transcription factors and forming large transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. HDAC2 also interacts in the late S-phase of DNA-replication with DNMT1 in transcriptional repressor complex composed of DNMT1, DMAP1, PCNA and CAF1.

In addition to histones, HDAC2 can deacetylate transcription factors and modify their transcriptional activity. HDAC2 has been shown to negatively regulate synaptic plasticity and memory formation. Elevated activity of HDAC2 has been known to associate with the loss of memory and neuronal degeneration. 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence derived from the C-terminus of the human HDAC2 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
IHC-P, WB - Verified 

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:200 by volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested dilution is 1:100 - 1:500. For Western blot the suggested dilution is 0.1 - 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only verified by western blot with the purified format.

Additional Product Notes

25 µg and 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-10 or 10-40 immunoprecipitations, respectively, at the recommended dilutions.

RRID
AB_2650674 (BioLegend Cat. No. 815203)
AB_2650660 (BioLegend Cat. No. 815204)

Antigen Details

Structure
488 amino acids with a predicted molecular weight of 55.4 kD. It contains a histone deacetylase domain.
Distribution

Widely expressed in many cell types; nucleus.

Function
HDAC2 is a histone deacetylase responsible for the deacetylation of ε-amino acid group of lysine residues on the N-terminal tail of core histones (H2A, H2B, H3, H4). HDAC2 forms complex with transcription factors and acts as a transcriptional co-repressor.
Interaction
Forms histone deacetylase complexes with HDAC1, RBBP4, and RBBP7. Interacts with GFI1, SNW1, HDAC7, PRDM6, SAP30, SETDB1, SUV39H1, H2AFY, ATR, CBFA2T3, BCL-6, DNMT1, MINT, HDAC10, HCFC1, NRIP1, KDM4A, TSHZ3 and PELP1.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Winter M, et al. 2013. EMBO J. 32:3176.
2. Ma P, et al. 2013. PLoS Genet. 9:e1003377.
3. Kurita M, et al. 2012. Nat. Neurosci. 15:1245.
4. Segre CV, et al. 2011. J. Biomed. Biotechnol. 2011:690848.
5. Brunmeir R, et al. 2009. Int. J. Dev. Biol. 53:275.
6. Guan JS, et al. 2009. Nature 459:55.

Gene ID
3066 View all products for this Gene ID
UniProt
View information about HDAC2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03.12.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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