- 3F3/HDAC2 (See other available formats)
- Other Names
- Histone deacetylase 2, YY1-associated factor 1, transcriptional regulator homolog RPD3, YAF1
- Mouse IgG1, κ
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- Product Citations
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. The purified format (Cat. No. 815201) can be used as an alternate.
HDAC2 is a member of class I histone deacetylase (HDACs) family, which modulates the chromatin structure by removing acetyl groups (deacetylation) from the side chain of lysine residues on the N-terminal region of core histones (H2A, H2B, H3 and H4). Generally, histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.
HDAC2 lacks DNA binding activity and executes its function by recruiting transcription factors and forming large transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. HDAC2 also interacts in the late S-phase of DNA-replication with DNMT1 in transcriptional repressor complex composed of DNMT1, DMAP1, PCNA and CAF1.
In addition to histones, HDAC2 can deacetylate transcription factors and modify their transcriptional activity. HDAC2 has been shown to negatively regulate synaptic plasticity and memory formation. Elevated activity of HDAC2 has been known to associate with the loss of memory and neuronal degeneration.
- Human, Mouse, Rat, Drosophila
- Antibody Type
- Host Species
- This monoclonal antibody was raised against a peptide sequence derived from the C-terminus of the human HDAC2 protein.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ChIP - Quality tested
IHC-P, WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:200 by volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested dilution is 1:100 - 1:500. For Western blot the suggested dilution is 0.1 - 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Reactivity to Drosophila was only verified by western blot with the purified format.
- Additional Product Notes
25 µg and 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-10 or 10-40 immunoprecipitations, respectively, at the recommended dilutions.
AB_2650674 (BioLegend Cat. No. 815203)
AB_2650660 (BioLegend Cat. No. 815204)
- 488 amino acids with a predicted molecular weight of 55.4 kD. It contains a histone deacetylase domain.
Widely expressed in many cell types; nucleus.
- HDAC2 is a histone deacetylase responsible for the deacetylation of Îµ-amino acid group of lysine residues on the N-terminal tail of core histones (H2A, H2B, H3, H4). HDAC2 forms complex with transcription factors and acts as a transcriptional co-repressor.
- Forms histone deacetylase complexes with HDAC1, RBBP4, and RBBP7. Interacts with GFI1, SNW1, HDAC7, PRDM6, SAP30, SETDB1, SUV39H1, H2AFY, ATR, CBFA2T3, BCL-6, DNMT1, MINT, HDAC10, HCFC1, NRIP1, KDM4A, TSHZ3 and PELP1.
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
- Antigen References
1. Winter M, et al. 2013. EMBO J. 32:3176.
2. Ma P, et al. 2013. PLoS Genet. 9:e1003377.
3. Kurita M, et al. 2012. Nat. Neurosci. 15:1245.
4. Segre CV, et al. 2011. J. Biomed. Biotechnol. 2011:690848.
5. Brunmeir R, et al. 2009. Int. J. Dev. Biol. 53:275.
6. Guan JS, et al. 2009. Nature 459:55.
- Gene ID
- 3066 View all products for this Gene ID
- View information about HDAC2 on UniProt.org
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