- 2F5A22 (See other available formats)
- Regulatory Status
- Other Names
- REL, V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog, Proto-Oncogene C-Rel
- Mouse IgG2b, κ
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- Product Citations
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c-Rel is a member of the Rel/NF-κB transcription factor family that forms homo or heterodimers with family members such as RelA/p65, RelB, NF-κB1/p105, and NF-κB2/p100. The NF-κB complex is retained in cytoplasm by the inhibitor IκB protein in unstimulated cells. Upon activation, IκB is degraded via the ubiquitin-proteasome pathway, which results in nuclear translocation of the active NF-κB complex. While other NF-κB transcription factors are expressed in most cell types, relatively higher levels of c-REL expression are found in hematopoietic cells. c-REL is thought to play key roles in regulating proliferation, differentiation, and survival of B and T lymphocytes.Product Details
- Antibody Type
- Host Species
- Partial c-REL recombinant protein (453-587 a.a.)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ChIP - Quality tested
WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml.
It is recommended that the reagent to be titrated for optimal performance before each experiment.
- Application Notes
25 µg, 100 µg of Go-ChIP-GradeTM Purified Antibody can be used for 2-4, 11-16 immunoprecipitations, respectively, at the recommended dilution.
AB_2687119 (BioLegend Cat. No. 655803)
AB_2687120 (BioLegend Cat. No. 655804)
- 619 amino acids, predicted molecular weight of 69 kD, contains a RHD (Rel-like) domain responsible for dimerization and DNA binding, contains two C-terminal transactivation domains
Cytoplasm, translocated to the nucleus upon activation
- Transcription factor belonging to the NF-κB family, regulates various biological processes including tumorgenesis, inflamation, immune response, cell proliferation, and differentiation
- Forms homo or heterodimer with other NF-κB family members; interacts with NKIRAS1, NFKBIB, NFKBIE, IRF-8, and Foxp3
- Cell Type
- B cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers, TCRs
- Antigen References
1. Bash J, et al. 1997. Mol. Cell Biol. 17:6526.
2. Chen G, et al. 2010. J. Immunol. 185:2350.
3. Gilmore TD, et al. 2011. Genes Cancer 2:695.
4. Ferch U, et al. 2007. Nat. Immunol. 8:984.
5. Sanjabi S, et al. 2005. Genes Dev. 19:2138.
6. Yamazaki T, et al. 2003. Nat. Immunol. 4:780.
- Gene ID
- 5966 View all products for this Gene ID
- View information about c-REL on UniProt.org
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