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GMP Recombinant Human IL-21 (carrier-free)

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Other Names
Interleukin 21, Interleukin-21, IL21
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GMP recombinant human IL-21 induces dose-dependent secretion of IFN-γ by NK-92 cells. ELISA MAX™ Deluxe Set Human IFN-γ (Cat No 430104) was used to measure IFN-γ. The ED50 range for this effect is 0.6 - 3.0 ng/mL.
  • Human_IL-21_CF_GMP-RECOM_1_072321_updated.png
    GMP recombinant human IL-21 induces dose-dependent secretion of IFN-γ by NK-92 cells. ELISA MAX™ Deluxe Set Human IFN-γ (Cat No 430104) was used to measure IFN-γ. The ED50 range for this effect is 0.6 - 3.0 ng/mL.
  • Human_IL-21_CF_GMP-RECOM_2_072321_updated.png
    Stability Testing for GMP Recombinant Human IL-21. GMP recombinant human IL-21 was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in an IFN-γ secretion assay with NK-92 cells.
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571216 100 µg 1875€
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571214 25 µg 563€
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Description

IL-21 was identified by a functional screening from conditioned medium of activated T cells using Baf3-IL-21R transfectants. In normal B cells, IL-21 can mediate cell proliferation, growth arrest, terminal differentiation, or apoptosis, depending on their activation status. IL-21 enhances B-cell proliferation following incubation with an activating CD40 antibody. This result suggests that IL-21 enhances B-cell function following T:B cell interactions. Nevertheless, IL-21 inhibits the proliferation of murine and human B cells stimulated with anti-IgM antibodies. In addition, IL-21 enhances proliferation, cytotoxic activity, and IFN-γ production by CD8-effector T cells, and induces terminal differentiation of activated natural killer (NK) cells. Also, IL-21 can drive Th17 responses in conjunction with TGF-β. Nevertheless, IL-21 is not essential for the differentiation of Th17 cells in vitro or in vivo, as it was shown using IL-21 and IL-21R-deficient mice. IL-6 induces the production of IL-21 from CD4 T cells upon TCR stimulation. In addition, IL-6 and IL-21 are key players in the Tfh differentiation, characterized by increased protein expression of both Bcl-6 and CXCR5. IL-21R complex is formed with the IL-21Rα chain and the common subunits (γc) shared with other interleukins, such as IL-2, IL-4, IL-7, IL-9, and IL-15.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human IL-21, amino acid Gln30-Ser162 (Accession # NM_021803), was expressed in E.coli.
Molecular Mass
The 133 amino acid recombinant protein has a predicted molecular mass of approximately 15.5 kD. The DTT-reduced and non-reduced protein migrate at approximately 16 kD by SDS-PAGE. The N-terminal amino acid is Glutamine.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.1 µm filtered protein solution is in PBS, 1 mM EDTA, pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method
Concentration
500 µg/mL
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.6 - 3.0 ng/mL as measured by its ability to induce dose-dependent secretion of IFN-γ by NK-92 cells. ELISA MAX™ Deluxe Set Human IFN-γ (Cat. No. 430104) was used to measure IFN-γ secretion.
Application

Bioassay
Cell Culture
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Disclaimer

GMP Recombinant Proteins. BioLegend GMP recombinant proteins are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

BioLegend GMP recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12.

Antigen Details

Structure
Interleukin
Distribution

CD4 activated T cells, Th17 cells, NK T cells

Function
IL-21 induces T- and B-cell proliferation, B-cell Ig class switching to IgG production, decreases dendritic cell function, enhances differentiation of effector and central memory T cells, and regulates T-cell and hematopoietic progenitor cell homeostasis.
Interaction
T cells, B cells, NK cells, and dendritic cells as well as some nonimmune cells, such as fibroblasts and epithelial cells
Ligand/Receptor
IL-21R and the common γc subunit (CD132) are components of the IL-21R complex.
Bioactivity
Measured by its ability to induce IFN-γ secretion by NK-92 cells.
Cell Type
B cells, Dendritic cells, Hematopoietic stem and progenitors, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Parrish-Novak J, et al. 2000. Nature. 408:57.
  2. De Totero D, et al. 2006. Blood. 107:3708.
  3. Korn T, et al. 2007. Nature. 448:484.
  4. Coquet JM, et al. 2008. J. Immunol. 180:7097.
  5. Dienz O, et al. 2009. J. Exp. Med. 206:69.
  6. Kaplan MH, et al. 2011. Blood. 117:6198.
  7. Eto D, et al. 2011. PLoS One. 6:e17739.
Gene ID
59067 View all products for this Gene ID
UniProt
View information about IL-21 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.22.2020

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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