- Other Names
- Granulocyte-Colony Stimulating Factor, CSF3, CSF-β
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- Product Citations
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Human G-CSF was cloned from a human squamous carcinoma cell line and is the key hematopoietic cytokine involved in the control of neutrophil production. Therefore, it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by down-regulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrade important HSC trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells; it is also expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells.Product Details
- Human G-CSF, amino acids Thr27-Pro200 (Accession# BC033245.1) was expressed in 293E cells.
- Molecular Mass
- The 174 amino acid recombinant protein has a predicted molecular mass of 18.6 kD. The DTT-reduced and the non-reduced proteins migrate at approximately 20 kD by SDS-PAGE. The N-terminal amino acid is Thr.
- > 95%, as determined by Coomassie stained SDS-PAGE
- 0.1 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method
- 500 µg/mL
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- ED50 = 0.02 - 0.08 ng/mL as measured by its ability to induce proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at email@example.com.
GMP Recombinant Proteins. BioLegend GMP recombinant proteins are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
BioLegend GMP recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12.
Hematopoietic stem and progenitors, embryonic stem cells
- G-CSF acts on myeloid progenitor cells to stimulate survival, proliferation, and neutrophilic maturation; on mature neutrophils, G-CSF enhances survival, superoxide anion and alkaline phosphatase production, arachidonic acid release, and antibody dependent cellular cytotoxicity.
- Hematopoietic progenitor cells, neutrophilic granulocytes, monocytes, platelets, endothelial cells, trophoblastic cells, myoblasts, cardiomyocytes, cardiac fibroblasts, neurons, and some small cell lung carcinoma cell line
- CD114 (G-CSFR)
- Measured by its ability to induce proliferation of M-NFS-60 cells
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
Nagata S, et al. 1986. Nature. 319:415.
Kim HK, et al. 2006. Blood. 108:812.
Donahue RE, et al. 2009. Blood. 114:2530.
Shimoji K, et al. 2010. Cell Stem Cell. 6:227.
Martins A, et al. 2010. IUBMB Life. 62:611.
Hara M, et al. 2011. J. Exp. Med. 208:715.
- Gene ID
- 1440 View all products for this Gene ID
- View information about G-CSF on UniProt.org