Fixation Buffer

Pricing & Availability
Other Names
Fixative, Paraformaldehyde
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420801 100 ml 40€
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Description

Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened paraformaldehyde with low background, thus producing the greatest signal to noise ratio.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
This buffer solution should be stored between 2°C and 8°C.
Application

ICFC - Quality tested
ICC

Recommended Usage

For cell fixation, use 0.5 ml fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.

Application Notes

This 1X PBS solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to perform phospho-flow in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  2. Kenna TJ, et al. 2010. J. Immunol. 184:598. PubMed
  3. Sullivan BP, et al. 2010. Am J Pathol. 177:2837. PubMed
  4. del Rio ML, et al. 2011.Transplantation. 92:1085. PubMed
  5. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
  6. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  7. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  8. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
Product Citations
  1. Sullivan B, et al. 2010. Am J Pathol. 177:2837. PubMed
  2. Kenna T, et al. 2010. J Immunol. 184:598. PubMed
  3. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  4. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  5. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  6. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  7. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
  8. Wright A, et al. 2015. Immunobiology. 220: 859-864. PubMed
  9. Clancy-Thompson E, et al. 2015. Cancer Immunol Res. 3: 956-967. PubMed
  10. Ede J, et al. 2015. Toxicol Sci. 148: 108 - 120. PubMed
  11. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
  12. Guye P, et al. 2016. Nat Commun. 7:10243. PubMed
  13. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  14. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  15. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  16. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  17. Bekeschus S, et al. 2016. J Leukoc Biol. 100: 791 - 799. PubMed
  18. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  19. Yokota-Nakatsuma A, et al. 2016. Sci Rep. 6:37914. PubMed
  20. Wang L, et al. 2016. Oncogene. 10.1038/onc.2016.380. PubMed
  21. Lamb C, et al. 2016. J Crohns Colitis. 10.1093/ecco-jcc/jjw189. PubMed
  22. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  23. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  24. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  25. Wilmes A, et al. 2017. Toxicol In Vitro. 10.1016/j.tiv.2017.07.023. PubMed
  26. Kuo T, et al. 2017. Oncogene. 10.1038/onc.2017.156. PubMed
  27. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  28. Melcher M, et al. 2017. Stem Cell Res Ther . 10.1186/s13287-017-0601-7. PubMed
  29. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  30. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  31. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  32. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  33. Gensterblum E,et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.09.011. PubMed
  34. Nouraei N,et al. 2017. Exp Neurol.. 10.1016/j.expneurol.2017.10.017. PubMed
Publication Library

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID
NA

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Go To Top Version: 3    Revision Date: 01.26.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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