FITC Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Fluorescein Isothiocyanate, SAv-FITC
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Product Citations
publications
SAV_FITC_082808.jpg
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC
  • SAV_FITC_082808.jpg
    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC
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Cat # Size Price Quantity Avail. Save
405201 100 µg 28,00€
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405202 500 µg 80,00€
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-fluorescein isothiocyanate (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-FITC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this Streptavidin-FITC is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the FITC conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Streptavidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-FITC is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Schmutz S, et al. 2009. J. Immunol. 183:2217. PubMed
  2. Cho HY, et al. 2009. Immunol Lett. 127:39. PubMed
  3. de Wispelaere D, et al. 2012. J. Virol. 86:7072. PubMed
  4. Fukuoka A, et al. 2013. Int Immunol. PubMed
  5. Giltiay NV, et al. 2013. J Exp Med. 25:373. PubMed
  6. Lee JH, et al. 2014. J Rheumatol. 41:2425. PubMed
Product Citations
  1. Sasaki N, Itakura Y, Toyoda M 2017. Stem Cell Res Ther. 10.1186/s13287-017-0534-1. PubMed
  2. Zhu Z, et al. 2017. Int J Biochem Cell Biol. 10.1016/j.biocel.2017.04.003. PubMed
  3. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  4. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  5. Hensel JA, et al. 2019. Mol Ther Oncolytics. 12:41. PubMed
  6. Mödinger Y, et al. 2018. J Cell Mol Med. 22:6002. PubMed
  7. Zhang Y, et al. 2019. JCI Insight. 4:2. PubMed
  8. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  9. Laurin M, et al. 2019. Elife. 8. PubMed
  10. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
  11. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  12. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  13. Schmutz S, et al. 2009. J Immunol. 183:2217. PubMed
  14. Lin Y, et al. 2012. PLoS One. 7:e30507. PubMed
  15. Yang M 2012. J Virol. 86:7072. PubMed
  16. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  17. Cho H, et al. 2009. Immunol Lett. 127:39. PubMed
  18. Giltiay N, et al. 2013. J Exp Med. 210:2773. PubMed
  19. Lee J, et al. 2014. J Rheumatol. 41:2425. PubMed
  20. Matt P, et al. 2015. PLoS One. 10: 0137474. PubMed
  21. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
  22. SL C, et al. 2016. JCI Insight. 1: 83116. PubMed
  23. Vigeland C, et al. 2016. PLoS One. 11: 0163288. PubMed
  24. Li S, et al. 2016. PLoS One. 11: 0163893. PubMed
  25. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  26. Geng S, et al. 2016. Nat Commun. 7:13436. PubMed

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Go To Top Version: 5    Revision Date: 06.17.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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