FITC anti-mouse CD4 Antibody

Pricing & Availability
H129.19 (See other available formats)
Regulatory Status
Other Names
L3T4, T4
Rat IgG2a, κ
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Product Citations
C57BL/6 splenocytes stained with H129.19 FITC
  • H129dot19_FITC_121508.jpg
    C57BL/6 splenocytes stained with H129.19 FITC
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130308 500 µg 194€
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Description CD4 is a 55 kD protein, also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck. The H129.19 antibody is able to block CD4 mediated cell adhesion and T cell activation. It is also useful for in vivo and in vitro depletion of CD4+ T cells. H129.19 antibody blocks binding of the anti-mouse CD4 mAbs GK1.5 and RM4-5, but not RM4-4 antibody. H129.19 antibody inhibits various responses of T helper cells to antigenic or mitogenic stimuli. Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Mouse lymphocytes
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1-3, depletion. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Naquet P. 1985. Immunogenetics. 22:247.
  2. Pierres A. 1984. J. Immunol.. 132:2775.
  3. Guo MW. 1995. Zygote. 3:65.
  4. Ghobrial RR. 1989. Clin Immunol Immunopathol. 52:486.
Product Citations
  1. Cai W, et al. 2020. J Immunol Res. 2019:2835256. PubMed
  2. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  3. Chaurasiya S, et al. 2020. Oncoimmunology. 9:1729300. PubMed
  4. Luo Z, et al. 2020. Front Immunol. 0.883333333. PubMed
  5. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
  6. Luo W, et al. 2019. Mol Med Rep. 20:1007. PubMed
  7. Ponzetta A, et al. 2020. Cell. 178(2):346-360.e24.. PubMed
  8. Chen S, et al. 2022. iScience. 25:105176. PubMed
  9. Koide S, et al. 2022. iScience. 25:103603. PubMed
  10. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  11. Terlizzi M, et al. 2021. Cell Physiol Biochem. 55:539. PubMed
AB_1279237 (BioLegend Cat. No. 130308)

Antigen Details

Ig superfamily, 55 kD
Majority of thymocytes, T cell subset
TCR co-receptor, T cell activation
MHC class II molecule
Cell Type
Thymocytes, T cells, Tregs
Biology Area
Molecular Family
CD Molecules
Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
4. Naquet P. 1985. Immunogenetics. 22:247.
5. Pierres A. 1984. J. Immunol.. 132:2775.
6. Guo MW. 1995. Zygote. 3:65-73.
7. Ghobrial RR. 1989. Clin Immunol Immunopathol. 52:486.
Gene ID
12504 View all products for this Gene ID
View information about CD4 on

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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