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FITC anti-human IgM Antibody

Pricing & Availability
Clone
MHM-88 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Mouse IgG1, κ
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Product Citations
publications
MHM-88_FITC_021009.jpg
Human peripheral blood lymphocytes stained with MHM-88 FITC
  • MHM-88_FITC_021009.jpg
    Human peripheral blood lymphocytes stained with MHM-88 FITC
Compare all formats See FITC spectral data
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314506 100 tests 151€
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Product Citations
  1. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
  2. Watanabe A, et al. 2019. Cell. 177:1124. PubMed
  3. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  4. Muir L, et al. 2018. Wellcome Open Res. 2:97. PubMed
  5. Fenwick C, et al. 2022. Nat Microbiol. 7:1376. PubMed
  6. Lindner JM, et al. 2019. Immunity. 50:668. PubMed
  7. Zost SJ, et al. 2020. Nat Med. 26:1422. PubMed
  8. Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
  9. Zost SJ, et al. 2020. bioRxiv. . PubMed
  10. Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
  11. Ros F, et al. 2020. MAbs. 12:1846900. PubMed
  12. Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed
  13. Lisboa F, et al. 2011. J Biol Chem. 286:7577. PubMed
  14. Nicolò A, et al. 2022. Front Immunol. 13:1016263. PubMed
  15. Benhammou JN, et al. 2020. Dig Dis Sci. 66:2394. PubMed
  16. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  17. Fenwick C, et al. 2021. Cell Rep. 37:109814. PubMed
  18. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
  19. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  20. Ju L,et al. 2017. Sci Rep.. 10.1038/s41598-017-13793-3. PubMed
  21. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  22. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
  23. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
RRID
AB_493009 (BioLegend Cat. No. 314506)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
3507 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 10.10.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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