FITC anti-human CX3CR1 Antibody

Pricing & Availability
2A9-1 (See other available formats)
Regulatory Status
Other Names
V28, GPR13, Chemokine C-X3-C receptor 1
Rat IgG2b, κ
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Product Citations
Human peripheral blood lymphocytes stained with 2A9-1 FITC and anti-CD3 (UCHT1) APC
  • 2A9-1_FITC_061109.jpg
    Human peripheral blood lymphocytes stained with 2A9-1 FITC and anti-CD3 (UCHT1) APC
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341605 25 tests 109€
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341606 100 tests 259€
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CX3CR1 is a G-protein-coupled seven-transmembrane chemokine receptor, also called GPR13 or V28. It is expressed on NK cells, T cell subset, monocytes/macrophages, dendritic cells, and some malignant epithelial cells. CX3CL1 (known also as fractalkine and neurotactin) is the ligand of CX3CR1. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediatesfirm cell adhesion and migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Reported Reactivity
African Green, Cynomolgus, Rhesus
Antibody Type
Host Species
CX3CR1-EGFP fusion protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Nishimura M, et al. 2002. J. Immunol. 168:6173.
  2. Nanki T, et al. 2002. Arthritis Rheum. 46:2878.
  3. Kobayashi T, et al. 2007. Inflamm. Bowel Dis. 13:837.
  4. Beziat V, et al. 2011. J. Immunol. 186:6753. PubMed.
Product Citations
  1. Healy LM, et al. 2020. Glia. 68:811. PubMed
  2. Fadini G, et al. 2014. Atherosclerosis. 237:805. PubMed
  3. Wragg KM, et al. 2020. Cell Reports. 31(11):107773. PubMed
  4. Staumont-Sallé D, et al. 2014. J Exp Med. 211:1185. PubMed
  5. Feuser K, et al. 2012. Cytokine. 58:178. PubMed
  6. Cavarelli M, et al. 2022. iScience. 25:104346. PubMed
  7. Kim M, et al. 2017. Cytokine. 10.1016/j.cyto.2017.07.007. PubMed
  8. Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed
  9. Bigler M, et al. 2015. PLoS One. 10: 0145635. PubMed
  10. Hasselmann J, et al. 2020. Neuron. 103(6):1016-1033. PubMed
  11. Zhao T, et al. 2012. PLoS One. 7:e43399. PubMed
  12. Scheiblich H, et al. 2021. Cell. 184:5089. PubMed
  13. Kleinnijenhuis J, et al. 2012. Proc Natl Acad Sci U S A. 109:17537. PubMed
  14. Berg P, et al. 2012. Clin Vaccine Immunol. 1.327777778. PubMed
  15. Remmerswaal E, et al. 2012. Blood. 119:1702. PubMed
  16. Lee CH, et al. 2018. Elife. 7:e32532. PubMed
  17. Verschoor C, et al. 2014. PLoS One. 9:104522. PubMed
AB_1626276 (BioLegend Cat. No. 341605)
AB_1626272 (BioLegend Cat. No. 341606)

Antigen Details

G-protein-coupled seven transmembrane receptor

NK cells, T cell subset, monocytes/macrophages, dendritic cells

cell adhesion and migration
CX3CL1 (Fractalkine, FKN, neurotactin)
Cell Type
Dendritic cells, Macrophages, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Imai T, et al. 1997. Cell. 91:521.
2. Fong AM, et al. 1998. J. Exp. Med. 188:1413.
3. Auffray C, et al. 2009. J. Exp. Med. 206:595.

Gene ID
1524 View all products for this Gene ID
View information about CX3CR1 on

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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