Direct-Blot™ HRP anti-IRF6 Antibody

Pricing & Availability
Clone
14B2C16 (See other available formats)
Other Names
Interferon regulatory factor 6, IRF-6
Isotype
Mouse IgG2a, κ
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Product Citations
publications
14B2C16_DBHRP_IRF6_Antibody_WB_080316
Total cell lysate from HaCaT cells (lane 1, 15 µg) and HeLa cells (lane 2, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with Direct-Blot™ HRP anti-IRF6 antibody (clone 14B2C16). Proteins were visualized using chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 14B2C16_DBHRP_IRF6_Antibody_WB_080316
    Total cell lysate from HaCaT cells (lane 1, 15 µg) and HeLa cells (lane 2, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with Direct-Blot™ HRP anti-IRF6 antibody (clone 14B2C16). Proteins were visualized using chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
Cat # Size Price Quantity Avail. Save
674505 100 µL 232€
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Description

IRF6 is a member of the IRF family of transcription factors, which are based on the presence of the conserved IRF tryptophan pentad repeat DNA-binding domain. Unlike other IRF transcription factors, IRF6 is not involved in the expression of interferon genes and doesn’t seem to be involved in innate immunity. Data suggests that IRF6 is necessary for appropriate epidermal differentiation and development. Mutations of IRF6 are associated with human craniofacial developmental defects such as cleft lip, palate, tooth agenesis, Van der Woude syndrome, and popliteal pterygium syndrome. IRF6-deficient mice display severe morphological defects in skin and limbs, and result in perinatal lethality as well.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human IRF6 recombinant protein (92-223 a.a.) expressed in E. coli.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

RRID
AB_2616953 (BioLegend Cat. No. 674505)

Antigen Details

Structure
467 amino acids with a predicted molecular weight of 53 kD and contains an IRF trytophan pentad repeat that is responsible for DNA binding.
Distribution

Nucleus and cytoplasm.

Function
IRF6 is a transcriptional activator that regulates proliferation and differentiation of both epithelial cells and keratinocytes. IRF6 is required for normal epidermal development during embryogenesis.
Interaction
Interacts with SERPINB5.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Melath A, et al. 2013. Asian Pac. J. Cancer Prev. 14:6803.
2. Salahshourifar I, et al. 2012. Eur. J. Med. Genet. 55:389.
3. Blackburn J, et al. 2012. Dev. Biol. 365:61.
4. Botti E, et al. 2011. Proc. Natl. Acad. Sci. USA 108:13710.
5. Restivo G, et al. 2011. EMBO 30:4571.
6. Fleming JA, et al. 2009. Mol. Cell Endocrinol. 299:252.

Gene ID
3664 View all products for this Gene ID
UniProt
View information about IRF6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08.03.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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