Western blot analysis of 15µg cell lysates from HeLa with or without 100 ng/mL Nocodazole treatment for 4 hours. Samples were resolved by electrophoresis (14% Tris Glycine gel), transferred to nitrocellulose, probed with 0.25 µg/mL Direct-Blot™ anti-Histone H3-Phosphorylated (Ser28) Antibody, clone HTA28. Go-ChIP-Grade™ Purified anti-Histone H3 (C-terminus) Antibody (Cat. No. 819404) was used as the loading control. Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
Western blot analysis of 15µg cell lysates from HeLa with or without 100 ng/mL Nocodazole treatment for 4 hours. Samples were resolved by electrophoresis (14% Tris Glycine gel), transferred to nitrocellulose, probed with 0.25 µg/mL Direct-Blot™ anti-Histone H3-Phosphorylated (Ser28) Antibody, clone HTA28. Go-ChIP-Grade™ Purified anti-Histone H3 (C-terminus) Antibody (Cat. No. 819404) was used as the loading control. Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability. During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase. H3 has been demonstrated to be phosphorylated by the action of MLTK-α (mixed linage kinase-like mitogen activated protein triple kinase α) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.
H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution
Nucleus
Function
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.
Interaction
Two molecules of H3 form a heterotetramer with two molecules of H4.
Biology Area
Cell Biology, DNA Repair/Replication, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References
1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545.
2. Goto H, et al. 2002. Genes Cells 7:11.
3. Garcia BA, et al. 2005. Biochemistry 44:13202.
Regulation
H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
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