Direct-Blot™ HRP anti-HDAC4 Antibody

Pricing & Availability
Clone
14A9C47 (See other available formats)
Other Names
Histone deacetylase 4, HD4
Isotype
Mouse IgG2b, κ
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Product Citations
publications
14A9C47_DB-HRP_HDAC4_Antibody_WB_042017
Total lysate (15µg protein) from human platelet (lane 1, negative control) and HeLa cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:1000 purified anti-HDAC4 or 1:1000, 1:2500 and 1:10000 Direct-Blot™ HRP anti- HDAC4 Antibody (upper). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for purified antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading controls (lower). Lane M is the MW ladder.
  • 14A9C47_DB-HRP_HDAC4_Antibody_WB_042017
    Total lysate (15µg protein) from human platelet (lane 1, negative control) and HeLa cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:1000 purified anti-HDAC4 or 1:1000, 1:2500 and 1:10000 Direct-Blot™ HRP anti- HDAC4 Antibody (upper). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for purified antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading controls (lower). Lane M is the MW ladder.
Cat # Size Price Quantity Avail. Save
676803 100 µL 232€
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Description

HDAC4 is a member of the class II histone deacetylase family and is similar to the yeast HDA1 protein. This nuclear protein operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC4 has been shown to play a crucial role in skeletal formation. HDAC4 prevents chondrocyte hypertrophy through inhibiting Runx2 activity during endochondral bone formation. The HDAC4 deficient mice show chondrocyte hypertrophy and premature ossification of developing bones. HDAC4 uses active nuclear export to shuttle between the nucleus and the cytoplasm. HDAC4 is modified by phosphorylation by CaM kinase I or IV. HDAC4 has been reported to interact with 14-3-3 in the cytoplasm and MEF2A, HDAC3, NcoR and SMRT in the nucleus.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human HDAC4 recombinant protein (529 - 651 a.a.) expressed in E. coli.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:5000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

Clone 14A9C47 weakly cross-reacts with mouse HDAC4.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2687040 (BioLegend Cat. No. 676803)

Antigen Details

Structure
1084 amino acids with a predicted molecular weight of 119 kD. Contains a histone deacetylase region and a N-terminal homodimerization domain.
Distribution

Cytoplasm and nucleus.

Interaction
CDKN2A forms a homodimer and interacts with HDAC7, MEF2C, BTBD14B, AHRR, NR2C1, adn 14-3-3.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Antigen References

1. Tong JK, et al. 1998. Nature 395:917.
2. Grunstein M.  1997. Nature 389:349.
3. Wolffe AP. 1996. Science 272:371.
4. Lee DY, et al. 1993. Cell 72:73.
5. Wang AH, et al. 2001. Mol. Cell Biol. 21:5992.
6. Bertos NR, et al. 1999. Mol. Cell Biol. 11:7816.

Gene ID
9759 View all products for this Gene ID
UniProt
View information about HDAC4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 04.21.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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