CD4 Fluorophore Sampler Kit with Veri-Cells™ PBMC

Pricing & Availability
Clone
RPA-T4 (See other available formats)
Other Names
T4, staining index, spillover spreading
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Product Citations
publications
CD4_Flurophore_Samplet_Kit_1_121217
Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA-T4) in each fluorophore conjugate separately.
  • CD4_Flurophore_Samplet_Kit_1_121217
    Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA-T4) in each fluorophore conjugate separately.
  • CD4_Flurophore_Samplet_Kit_2_121217
    Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA-T4) in each fluorophore conjugate separately.
  • CD4_Flurophore_Samplet_Kit_3_121217
    Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA-T4) in each fluorophore conjugate separately.
  • CD4_Flurophore_Samplet_Kit_4_121217
    Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA-T4) in each fluorophore conjugate separately.
Cat # Size Price Quantity Avail. Save
300562 1 kit 720€
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Description

The CD4 Fluorophore Sampler Kit contains 18 different fluorophore formats of clone RPA-T4 with 10 tests of each antibody per vial.  It can be useful to have the same clone in multiple fluorophore formats to enable instrument characterization and standardization. This kit also contains a vial of 25 tests of Veri-Cells™ PBMC.

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Chimpanzee, Cynomulgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Brilliant Violet™ conjugates: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
All other conjugates: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Storage & Handling
The kit should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Brilliant Violet 421™, Brilliant Violet 510™, Brilliant Violet 570™, Brilliant Violet 605™, Brilliant Violet 650™, Brilliant Violet 711™, Brilliant Violet 785™ are trademarks of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser
Violet Laser (405 nm)
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Red Laser (633 nm)
Application Notes

Protocol for using Veri-Cells™ PBMC:
1. Remove a vial of Veri-Cell™ PBMC from the refrigerator and warm to room temperature for 5 minutes.
2. Add 1.3ml of the Veri-Cell™ Buffer A to the cell pellet, replace the rubber stopper and mix by inversion for 5 seconds. Allow at least 5 minutes for the cells to rehydrate prior to staining.
3. Aliquot 50ul of cells into tubes. Stain each tube seperately with 5ul of each CD4 antibody conjugate per tube.

 

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, and blocking of T cell activation1,2.  This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300516).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

Antigen Details

Cell Type
Dendritic cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 1    Revision Date: 12.15.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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