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c-Myc Peptide Tag (Previously Covance catalog# PEP-150P)

Pricing & Availability
Regulatory Status
RUO
Other Names
(CEQKLISEEDL), Myc Proto-Oncogene Protein, Proto-Oncogene C-Myc, Transcription Factor P64, Class E Basic Helix-Loop-Helix Protein 39, Avian Myelocytomatosis Viral Oncogene Homolog, V-Myc, Myc-Related Translation/Localization
Previously
Covance Catalog# PEP-150P
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
931701 1 mg 171€
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931702 5 mg 347€
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Description

The c-myc tag is often used as a protein modification in order to simplify the labeling and detection of proteins. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to targeted proteins and allow for specific detection in western blotting, immunoprecipitation, and immunostaining techniques.

Product Details
Technical Data Sheet (pdf)

Product Details

Purity
>90% pure by HPLC
Formulation
Lyophilized.
Storage & Handling
Store lyophilized or at -20°C in solution
Application

IP, WB
Recommended Usage

Soluble in PBS. The optimal working dilution should be determined for each specific assay condition.
• Elution of epitope tag fusion proteins from immunoaffinity columns: 400 ug/mL
• Competitive inhibition of antibody in western or immunostaining: 10-fold molar excess over optimized (low) antibody

Application Notes

This synthetic peptide (H2N-CEQKLISEEDL-COOH) corresponds to the epitope detected by the 9E10 monoclonal antibody.

This peptide can be used in competition experiments, or to elute bound c-myc fusion proteins from the 9E10 affinity matrix.
 

Application References

(PubMed link indicates BioLegend citation)
  1. Enquist-Newman M, et al. 2001. Mol. Biol. Cell. 12: 2601.
  2. Ferrando A, et al. 2001. Nucleic Acids Res. 29:3685.

Related FAQs

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Go To Top Version: 2    Revision Date: 07.27.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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